Rad18 antibody (mAb)

for Rad18 antibody (mAb)Background

Rad18 (RNF73, hHR18) is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Rad18 is similar to its yeast counterpart, as it is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif.

for Rad18 antibody (mAb)Immunogen

This antibody was raised against a synthetic peptide corresponding to amino acid residues 402-414 of human Rad18.

for Rad18 antibody (mAb)Application Notes

This Rad18 antibody has been validated for use in Western blotting (1 - 2 µg/ml dilution). For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

for Rad18 antibody (mAb)Buffer

PBS containing 0.02% sodium azide. Sodium azide is highly toxic.

for Rad18 antibody (mAb)Positive Control

Active Motif's HL-60 nuclear extract (Catalog No. 36072) can be used as a positive control for Rad18 antibody.

for Rad18 antibody (mAb)Storage

Antibodies in solution can be stored at -80°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Rad18 antibody (mAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.