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Antibodies

BRD8 / SMAP2 antibody (pAb)

Aliases: SMAP, p120
Catalog No: 61007 Format: 100 µl $420 Buy Now
Catalog No: 61008 Format: 10 µl $95 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:145 kDa
Reactivity:Human

Applications

ChIP Validated ChIP-Seq Validated Western Blot Validated

for BRD8 / SMAP2 antibody (pAb)Application Notes


Validated Applications:
ChIP: 10 µl per ChIP
ChIP-Seq:10 µl each
WB: 1:500 - 1:1,000 dilution

ChIP-Seq validation was performed by Active Motif's Epigenetics Services and the complete data set is available in the UCSC Genome Browser by clicking here.

for BRD8 / SMAP2 antibody (pAb)Immunogen

This BRD8 / SMAP2 antibody was raised against a peptide corresponding to amino acids within the N-terminal region of human BRD8.

for BRD8 / SMAP2 antibody (pAb)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for BRD8 / SMAP2 antibody (pAb)References

 
BRD8 / SMAP2 antibody (pAb) tested by ChIP-Seq.

BRD8 / SMAP2 antibody (pAb) tested by ChIP-Seq.
ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 30 ug of chromatin from an adenocarcinoma cell line and 10 µl of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 20 million sequence tags were mapped to identify BRD8 / SMAP2 binding sites. The image shows binding across a region of chromosome 9. You can view the complete data set in the UCSC Genome Browser, starting at this specific location, here.

BRD8 / SMAP2 antibody (pAb) tested by Western blot.

Western blot of BRD8 / SMAP2 antibody.
Nuclear extract of HeLa cells (30 μg) probed with BRD8 / SMAP2 antibody (1:500).

for BRD8 / SMAP2 antibody (pAb)Background

BRD8 (Bromodomain-containing protein 8, SMAP2, TRCP120) is a member of the Bromodomain-containing family of chromatin associated proteins. BRD8 is a member of the NuA4 histone acetyltransferase complex, involved in the transcriptional activation of specific genes through the acetylation of histones H2A and H4. BRD8 contains two Bromodomains, a motif that has been demonstrated to facilitate interaction with acetylated histones.

for BRD8 / SMAP2 antibody (pAb)Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for BRD8 / SMAP2 antibody.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with BRD8 / SMAP2 antibody (pAb).

for BRD8 / SMAP2 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for BRD8 / SMAP2 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

BRD8 / SMAP2 antibody (pAb)
 

Data Thumbnails

BRD8 / SMAP2 antibody (pAb) tested by ChIP-Seq.

ChIP-Seq of BRD8 / SMAP2 pAb.
(Click image to enlarge and see details.)

BRD8 / SMAP2 antibody (pAb) tested by Western blot.

Western blot of BRD8 / SMAP2 pAb.
(Click image to enlarge and see details.)