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Antibodies

SET8 / PR-SET7 antibody (pAb)

Aliases: Lysine Methyltransferase 5A, SETD8, KMT5A, SET07
Catalog No: 61009 Format: 100 µl $410 Buy Now
Catalog No: 61010 Format: 10 µl $95 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:45 kDa
Reactivity:Human

Applications

Western Blot Validated

for SET8 / PR-SET7 antibody (pAb)Application Notes


Validated Applications:
WB: 1:500 - 1:1,000 dilution

for SET8 / PR-SET7 antibody (pAb)Immunogen

This SET8 / PR-SET7 antibody was raised against a peptide corresponding to amino acids within the N-terminal half of human SETD8.

for SET8 / PR-SET7 antibody (pAb)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for SET8 / PR-SET7 antibody (pAb)References

 
SET8 / PR-SET7 antibody (pAb) tested by Western blot.

Western blot of SET8 / PR-SET7 antibody.
Nuclear extract of HeLa cells (20 µg per lane) probed with SET8 / PR-SET7 antibody (1:500). Lane 1: No treatment. Lane 2: Cells treated with colcemid.

for SET8 / PR-SET7 antibody (pAb)Background

SET8 (SET-domain containing protein 8, PR-SET7, SETD8, KMT5A) is a SET domain-containing histone methyltransferase that specifically monomethylates histone H4 Lys20. H4 Lys20 monomethylation is enriched during mitosis and is associated with transcriptional repression. SET8 mainly functions in euchromatic regions, playing a role in gene silencing. SET8 knockout is homozyhgous lethal in mice and its function is required for cell cycle progression and cell proliferation. SET8 is required for proper chromosome condensation during interphase and chromosome segregation during mitosis. SET8 is also involved in DNA damage repair, as cells lacking SET8 function accumulate massive amounts of DNA damage. SET8 has been found to methylate p53 at Lys382, indicating a potential mechanism for SET8s role in the DNA damage repair pathway.

for SET8 / PR-SET7 antibody (pAb)Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for SET8 / PR-SET7 antibody.

for SET8 / PR-SET7 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for SET8 / PR-SET7 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

SET8 / PR-SET7 antibody (pAb)
 

Data Thumbnails

SET8 / PR-SET7 antibody (pAb) tested by Western blot.

Western blot of SET8 / PR-SET7 pAb.
(Click image to enlarge and see details.)