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Estrogen Receptor α antibody (mAb)
| Clone: F3 |
| Catalog No: 61035 | Format: 100 µg | $340 | Buy Now |
| Catalog No: 61036 | Format: 10 µg | $70 | Buy Now |
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| Western Blot | Chromatin IP | Immunofluorescence | Immunoprecipitation |
|---|---|---|---|
| Published. See application notes. |
Published. See application notes. |
Published. See application notes. |
Published. See application notes. |
for Estrogen Receptor-alpha antibody (mAb) (Clone F3)Background
ER-α (estrogen receptor α) is a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding and activation of transcription. ER-α localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer and osteoporosis.
for Estrogen Receptor-alpha antibody (mAb) (Clone F3)Immunogen
This ER-alpha antibody was raised against a peptide within the C-terminal region of human Estrogen Receptor alpha.
for Estrogen Receptor-alpha antibody (mAb) (Clone F3)Application Notes
This ER-α has been validated for use in EMSA, chromatin IP (ChIP), immunofluorescence, immunohistochemistry, immunoprecipitation and Western blotting. See references for more details.
for Estrogen Receptor-alpha antibody (mAb) (Clone F3)Buffer
Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 32 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide.
for Estrogen Receptor-alpha antibody (mAb) (Clone F3)Positive Control
Active Motif's T-47D nuclear extract (Catalog No. 36027) can be used as a positive control for ER-α antibody.
Chromatin IP Kits & Reagents
Active Motif has a variety of Chromatin Immunoprecipitation kits and reagents for every need. Please visit our Chromatin IP Page to learn about our ChIP kits, controls, chromatin and PCR primers for use with Estrogen Receptor α antibody (mAb).
Bridging Antibody to Improve ChIP and IP
Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP and IP experiments that use Estrogen Receptor α antibody (mAb).
Compatible Secondary Antibodies
Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Estrogen Receptor α antibody (mAb).
for Estrogen Receptor-alpha antibody (mAb) (Clone F3)References
This antibody has been used in the following references:
ChIP: Welboren WJ et al (2009) EMBO J 28: 1418-1428.
for Estrogen Receptor-alpha antibody (mAb) (Clone F3)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for Estrogen Receptor-alpha antibody (mAb) (Clone F3)Guarantee
This product is guaranteed for 6 months from date of arrival.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
