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Antibodies

Estrogen Receptor α antibody (mAb)

Clone: F3
Aliases: ESR1, ER, ESR, Era, ESRA, ESTRR, NR3A1
Catalog No: 61035 Format: 100 µg $410 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG1
Purification:Protein G Chromatography
Host:Mouse
Molecular Weight:66 kDa
Reactivity:Human

Applications

ChIP Validated Western Blot Validated Immunoprecipitation Validated

for Estrogen Receptor-a antibody (mAb) (Clone F3)Application Notes


Validated Applications:
WB: 1:500 - 1:1,000 dilution

Published Applications:
ChIP
WB

The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

for Estrogen Receptor-a antibody (mAb) (Clone F3)Immunogen

This ER-alpha antibody was raised against a peptide within the C-terminal region of human Estrogen Receptor alpha.

for Estrogen Receptor-a antibody (mAb) (Clone F3)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for Estrogen Receptor-a antibody (mAb) (Clone F3)References

 
Estrogen Receptor-α antibody (mAb) tested by ChIP.

Estrogen Receptor-α antibody (mAb) tested by ChIP.
Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 30 µg of MCF7 cell chromatin (treated with 10nM estradiol for 1 hour) and 10 µg of Estrogen Receptor-α antibody. ChIP DNA was used in qPCR with the negative control primer pairs or gene-specific primer pairs as indicated. Data are presented as Binding Events Detected per 1000 Cells using Active Motif's Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.

for Estrogen Receptor-a antibody (mAb) (Clone F3)Background

ER-α (estrogen receptor α) is a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding and activation of transcription. ER-α localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer and osteoporosis.

for Estrogen Receptor-a antibody (mAb) (Clone F3)Positive Control

Active Motif's T-47D nuclear extract (Catalog No. 36027) can be used as a positive control for ER-α antibody.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Estrogen Receptor α antibody (mAb).

Bridging Antibody to Improve ChIP, MeDIP and IP

Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use Estrogen Receptor α antibody (mAb).

for Estrogen Receptor-a antibody (mAb) (Clone F3)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Estrogen Receptor-a antibody (mAb) (Clone F3)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Estrogen Receptor α antibody (mAb)
 

Data Thumbnails

Estrogen Receptor-α antibody (mAb) tested by ChIP.

ChIP of Estrogen Receptor-α mAb.
(Click image to enlarge and see details.)