Your Cart
Browse
Filter
Estrogen Receptor α antibody (mAb)
| Clone: F3 |
| Catalog No: 61035 | Format: 100 µg | $340 | Buy Now |
| Catalog No: 61036 | Format: 10 µg | $70 | Buy Now |
|
|
Applications Table
| Western Blot | Chromatin IP | IF | IP |
|---|---|---|---|
| Published. See Application Notes below. |
Published. See Application Notes below. |
Published. See Application Notes below. |
Published. See Application Notes below. |
for Estrogen Receptor-alpha antibody (mAb) (Clone F3)Background
ER-α (estrogen receptor α) is a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding and activation of transcription. ER-α localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer and osteoporosis.
for Estrogen Receptor-alpha antibody (mAb) (Clone F3)Immunogen
This ER-alpha antibody was raised against a peptide within the C-terminal region of human Estrogen Receptor alpha.
for Estrogen Receptor-alpha antibody (mAb) (Clone F3)Application Notes
This ER-α has been validated for use in EMSA, chromatin IP (ChIP), immunofluorescence, immunohistochemistry, immunoprecipitation and Western blotting. See references for more details.
for Estrogen Receptor-alpha antibody (mAb) (Clone F3)Buffer
Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 32 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide.
for Estrogen Receptor-alpha antibody (mAb) (Clone F3)Positive Control
Active Motif's T-47D nuclear extract (Catalog No. 36027) can be used as a positive control for ER-α antibody.
Chromatin IP Assays
Active Motif has a variety of Chromatin Immunoprecipitation Kits for every need.
Please visit our Chromatin IP Page to learn about ChIP products designed for use with Estrogen Receptor α antibody (mAb).
Bridging Antibody to Improve ChIP, MeDIP and IP
Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use Estrogen Receptor α antibody (mAb).
Compatible Secondary Antibodies
Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Estrogen Receptor α antibody (mAb).
for Estrogen Receptor-alpha antibody (mAb) (Clone F3)References
This antibody has been used in the following references:
ChIP: Welboren WJ et al (2009) EMBO J 28: 1418-1428.
for Estrogen Receptor-alpha antibody (mAb) (Clone F3)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for Estrogen Receptor-alpha antibody (mAb) (Clone F3)Guarantee
This product is guaranteed for 6 months from date of arrival.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
