BLM antibody (pAb)

for BLM antibody (pAb)Background

BLM (Bloom Syndrome protein, RECQ2, RECQL3) is a DNA helicase similar to E. coli RecQ protein that is mutated in patients with Bloom Syndrome, an autosomal recessive disorder characterized by growth deficiency, sun sensitive skin, cancer predisposition and chromosomal instability. The five human RecQ helicases play critical roles in DNA recombination, replication and repair. BLM helps to resolve aberrant chromosome structures generated during DNA replication. It exists in a complex with topoisomerase IIIα, RMI1/BLAP75 and RMI2 and it is thought that BLM complexes are recruited to resolve Holliday junction intermediates. BLM is heavily phosphorylated, especially during mitosis. BLM has a role during meiosis as well, as BLM males are sterile and BLM females have reduced fertility.

for BLM antibody (pAb)Immunogen

This BLM antibody was raised against a peptide within the N-terminal region of human BLM.

for BLM antibody (pAb)Application Notes

This BLM antibody has been validated for use in Western blot (1:500 - 1:1,000).

for BLM antibody (pAb)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 32 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide.

for BLM antibody (pAb)Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for BLM antibody.

for BLM antibody (pAb)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for BLM antibody (pAb)Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.