GATA-6 antibody (pAb)

RRID: AB_2793493
Catalog No: 61063 Format: 100 µl $500 Buy
Catalog No: 61064 Format: 10 µl $125 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
62 kDa
Reactivity:
Human

Applications

Western Blot Validated Immunofluorescence Validated Immunocytochemistry Validated

Application Notes

Applications Validated by Active Motif:
ICC/IF: 1:2,000 dilution
WB: 1:500 dilution

Immunogen

This GATA-6 antibody was raised against a peptide within the N-terminus of human GATA-6.

Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

 

GATA-6 antibody (pAb) tested by Immunofluorescence.
Formaldehyde fixed HeLa cells stained with GATA-6 antibody at a 1:2,000 dilution.

GATA-6 antibody (pAb) tested by Western blot.
Nuclear extract (20 µg) of SW48 cells probed with GATA-6 antibody at a dilution of 1:500.

Background

GATA-6 (GATA binding factor 6) is a Zn-finger DNA binding transcription factor that is required for the expression of genes regulating formation of the primitive endoderm lineage. GATA-6, along with GATA-4, is a marker of extraembryonic endoderm.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting