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Antibodies

GATA-6 antibody (pAb)

Catalog No: 61063 Format: 100 µl $410 Buy Now
Catalog No: 61064 Format: 10 µl $95 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:62 kDa
Reactivity:Human

Applications

Western Blot Validated Immunofluorescence Validated Immunocytochemistry Validated

for GATA-6 antibody (pAb)Application Notes


Validated Applications:
ICC/IF: 1:2,000 dilution
WB: 1:500 dilution

for GATA-6 antibody (pAb)Immunogen

This GATA-6 antibody was raised against a peptide within the N-terminus of human GATA-6.

for GATA-6 antibody (pAb)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for GATA-6 antibody (pAb)References

 
GATA-6 antibody (pAb) tested by immunofluorescence.

GATA-6 antibody (pAb) tested by Immunofluorescence.
Formaldehyde fixed HeLa cells stained with GATA-6 antibody at a 1:2,000 dilution.

GATA-6 antibody (pAb) tested by Western blot.

GATA-6 antibody (pAb) tested by Western blot.
Nuclear extract (20 µg) of SW48 cells probed with GATA-6 antibody at a dilution of 1:500.

for GATA-6 antibody (pAb)Background

GATA-6 (GATA binding factor 6) is a Zn-finger DNA binding transcription factor that is required for the expression of genes regulating formation of the primitive endoderm lineage. GATA-6, along with GATA-4, is a marker of extraembryonic endoderm.

for GATA-6 antibody (pAb)Positive Control

Active Motif's SW48 nuclear extract (Catalog No. 36061) can be used as a positive control for GATA-6 antibody.

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with GATA-6 antibody (pAb).

for GATA-6 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for GATA-6 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

GATA-6 antibody (pAb)
 

Data Thumbnails

GATA-6 antibody (pAb) tested by immunofluorescence.

Immunofluorescence stain of GATA-6 pAb.
(Click image to enlarge and see details.)

GATA-6 antibody (pAb) tested by Western blot.

Western blot of GATA-6 pAb.
(Click image to enlarge and see details.)