RAP1 / TERF2IP antibody (pAb)

for RAP1 / TERF2IP antibody (pAb)Background

RAP1 / TERF2IP (Repressor/activator protein 1, Telomeric repeat-binding factor 2-interacting protein 1) is a protein associated with telomeres that acts both as a modulator of telomere function and as a regulator of transcription. RAP1 is a component of the shelterin (telosome) protein complex and is involved in the regulation of telomere length and serves to protect telomeric repeats from degradation. In contrast to other components of the shelterin complex, RAP1 is dispensable for telomere end-capping and does not participate in the protection of telomeres against non-homologous end-joining (NHEJ)-mediated repair. Instead, it is required to negatively regulate telomere recombination and is essential for repressing homology-directed repair (HDR), which can affect telomere length. RAP1 is recruited to telomeric repeats via its interaction with TERF2. Independent of its function in telomeres, RAP1 also acts as a transcriptional regulator. It is recruited to non-telomeric 5'-TTAGGG-3' sites via its association with TERF2 or other factors, and regulates gene expression. RAP1 is also present in the cytoplasm and associates with the IκB-kinase (IKK) complex and acts as a regulator of the NFκB signaling by promoting IKK-mediated phosphorylation of RELA/p65, leading to activation of the expression of NFκB target genes.

for RAP1 / TERF2IP antibody (pAb)Immunogen

This RAP1 / TERF2IP antibody was raised against a peptide within human RAP1 / TERF2IP.

for RAP1 / TERF2IP antibody (pAb)Application Notes

This RAP1 / TERF2IP antibody has been validated for use in Western blot (1:500 - 1:2,000).

for RAP1 / TERF2IP antibody (pAb)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 32 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide.

for RAP1 / TERF2IP antibody (pAb)Positive Control

Active Motif's K-562 nuclear extract (Catalog No. 36015) can be used as a positive control for RAP1 / TERF2IP antibody.

for RAP1 / TERF2IP antibody (pAb)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for RAP1 / TERF2IP antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of arrival.