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Antibodies

c-Myc antibody (mAb)

Clone: 6A10
Catalog No: 61075 Format: 100 µg $370 Buy Now
Catalog No: 61076 Format: 10 µg $80 Buy Now
Antibody Type:Monoclonal
Host:Rat
Isotype:IgG2a
Purification:Protein G Chromatography
Applications:EMSA, IP, WB
Reactivity:Human
Molecular Weight:68 kDa

Applications Table

Western Blot Chromatin IP Immunoprecipitation
Published.
See Application Notes below.
Published.
See Application Notes below.

for c-Myc antibody (mAb) (Clone 6A10)Immunogen

This c-Myc antibody was raised against a recombinant protein corresponding to amino acids 1-262 of human c-Myc protein.

for c-Myc antibody (mAb) (Clone 6A10)Application Notes

This c-Myc antibody has been validated for use in EMSA, immunoprecipitation and Western blotting. See References for details.

for c-Myc antibody (mAb) (Clone 6A10)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for c-Myc antibody (mAb) (Clone 6A10)References

This antibody has been used in the following references:
EMSA: Sommer A et al. (1998) J Biol Chem 273: 6632-6642.
IP: Vervoorts J et al. (2003) EMBO Reports 4: 484-490.
WB: Vervoorts J et al. (2003) EMBO Reports 4: 484-490.

for c-Myc antibody (mAb) (Clone 6A10)Background

c-Myc (Myc) is a sequence specific DNA binding transcription factor that regulates gene expression, especially genes involved in cell division. Myc family members contain a basic Helix-Loop-Helix leucine zipper (bHLHZ) domain. Myc family proteins associated with thousands of genes, especially those involved in cell growth and proliferation. c-Myc serves as an integrator of signal transduction, linking extracellular signals to an acute cellular growth response. Mutations and overexpression of c-Myc are associated with cell proliferation and cancer.

for c-Myc antibody (mAb) (Clone 6A10)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for c-Myc antibody (mAb) (Clone 6A10)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FACS = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

c-Myc antibody (mAb)