RNA pol II antibody (mAb)

for RNA pol II antibody (mAb) (Clone 4F8)Background

RNA pol II (RNA polymerase II) is responsible for synthesizing messenger RNA in eukaryotes. RNA pol II contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, RNA pol II, in combination with several other polymerase subunits, form the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.

for RNA pol II antibody (mAb) (Clone 4F8)Immunogen

This RNA pol II antibody was raised against a synthetic peptide corresponding to human RNA pol II C-terminal heptad repeat domain.

for RNA pol II antibody (mAb) (Clone 4F8)Application Notes

This RNA pol II antibody has been validated for use in chromatin IP (ChIP), immunofluorescence, immunoprecipitation and Western blotting. See references for more details.

for RNA pol II antibody (mAb) (Clone 4F8)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 32 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide.

for RNA pol II antibody (mAb) (Clone 4F8)Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for RNA pol II antibody.

for RNA pol II antibody (mAb) (Clone 4F8)References

This antibody has been used in the following references:
ChIP: Chapman RD et al (2007) Science 318: 1780-1782.
IF: Chapman RD et al (2007) Science 318: 1780-1782.
IP: Chapman RD et al (2007) Science 318: 1780-1782.
WB: Chapman RD et al (2007) Science 318: 1780-1782.

for RNA pol II antibody (mAb) (Clone 4F8)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for RNA pol II antibody (mAb) (Clone 4F8)Guarantee

This product is guaranteed for 6 months from date of arrival.