RNA pol II CTD phospho Ser7 antibody (mAb)

for RNA pol II CTD phospho Ser7 antibody (mAb) (Clone 4E12)Background

RNA pol II (RNA polymerase II) is responsible for synthesizing messenger RNA in eukaryotes. RNA pol II contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, RNA pol II, in combination with several other polymerase subunits, form the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.

During the transcription cycle, the CTD of the large subunit of RNA pol II is reversibly phosphorylated. RNA pol II containing unphosphorylated CTD is recruited to the promoter, whereas the hyperphosphorylated CTD form is involved in active transcription. Phosphorylation occurs at two sites within the heptapeptide repeat, at serine 2, serine 5 and serine 7. RNA pol II Serine 7 phosphorylation is confined to promoter regions and is necessary for the initiation of transcription.

for RNA pol II CTD phospho Ser7 antibody (mAb) (Clone 4E12)Immunogen

This RNA pol II CTD phospho Ser7 antibody was raised against a peptide containing the RNA pol II CTD sequence phosphorylated at serine 7.

for RNA pol II CTD phospho Ser7 antibody (mAb) (Clone 4E12)Application Notes

This RNA pol II phospho Ser7 antibody has been validated for use in chromatin IP (ChIP), immunofluorescence, immunoprecipitation and Western blotting. See references for more details.

for RNA pol II CTD phospho Ser7 antibody (mAb) (Clone 4E12)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 32 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide.

for RNA pol II CTD phospho Ser7 antibody (mAb) (Clone 4E12)Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for RNA Pol II CTD phospho Ser7 antibody.

for RNA pol II CTD phospho Ser7 antibody (mAb) (Clone 4E12)References

This antibody has been used in the following references:
ChIP: Chapman RD et al (2007) Science 318: 1780-1782.
IF: Chapman RD et al (2007) Science 318: 1780-1782.
IP: Chapman RD et al (2007) Science 318: 1780-1782.
WB: Chapman RD et al (2007) Science 318: 1780-1782.

for RNA pol II CTD phospho Ser7 antibody (mAb) (Clone 4E12)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for RNA pol II CTD phospho Ser7 antibody (mAb) (Clone 4E12)Guarantee

This product is guaranteed for 6 months from date of arrival.