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Antibodies

BMI-1 antibody (mAb)

Clone: DC9
Aliases: PCGF4, RNF51, FLVI2/BMI1
Catalog No: 61089 Format: 100 µg $410 Buy Now
Catalog No: 61090 Format: 10 µg $95 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG1k
Purification:Protein G Chromatography
Host:Mouse
Molecular Weight:40 kDa
Reactivity:Human, Mouse

Applications

Western Blot Validated Immunofluorescence Validated Immunocytochemistry Validated

for BMI-1 antibody (mAb) (Clone DC9)Application Notes


Validated Applications:
ICC/IF: 0.5 µg/ml dilution

Published Applications:
WB
See references for more information. Individual optimization may be required.

for BMI-1 antibody (mAb) (Clone DC9)Immunogen

This BMI-1 antibody was raised against a recombinant protein corresponding to full-length mouse BMI-1.

for BMI-1 antibody (mAb) (Clone DC9)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for BMI-1 antibody (mAb) (Clone DC9)References

 
BMI-1 antibody (mAb) tested by immunofluorescence.

BMI-1 antibody (mAb) tested by Immunofluorescence.
Formaldehyde fixed HeLa cells stained with BMI-1 antibody at a 0.5 µg/ml dilution.

for BMI-1 antibody (mAb) (Clone DC9)Background

BMI-1 is a member of the Polycomb PRC1 complex that is recruited to transcriptionally repressed genes subsequent to histone H3 lysine 27 methylation in order to maintain repression. In the PRC1 complex, BMI-1 is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2, resulting in the ubiquitylation of histone H2A at lysine 119. BMI-1 acts as an oncogene and cooperates with c-myc in the initiation of lymphoma. High levels of expression of BMI-1 are observed in metastatic melanoma, and BMI-1 has been implicated in several other cancers. BMI-1 is required for hematopoietic stem cell self renewal.

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with BMI-1 antibody (mAb).

for BMI-1 antibody (mAb) (Clone DC9)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for BMI-1 antibody (mAb) (Clone DC9)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

BMI-1 antibody (mAb)
 

Data Thumbnails

BMI-1 antibody (mAb) tested by immunofluorescence.

Immunofluorescence stain of BMI-1 mAb.
(Click image to enlarge and see details.)