BMI-1 antibody (mAb)

for BMI-1 antibody (mAb) (Clone DC9)Background

BMI-1 is a member of the Polycomb PRC1 complex that is recruited to transcriptionally repressed genes subsequent to histone H3 lysine 27 methylation in order to maintain repression. In the PRC1 complex, BMI-1 is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2, resulting in the ubiquitylation of histone H2A at lysine 119. BMI-1 acts as an oncogene and cooperates with c-myc in the initiation of lymphoma. High levels of expression of BMI-1 are observed in metastatic melanoma, and BMI-1 has been implicated in several other cancers. BMI-1 is required for hematopoietic stem cell self renewal.

for BMI-1 antibody (mAb) (Clone DC9)Immunogen

This BMI-1 antibody was raised against a recombinant protein corresponding to full length mouse BMI-1.

for BMI-1 antibody (mAb) (Clone DC9)Application Notes

This BMI-1 antibody has been validated for use in Western blot and immunofluorescence. See references for details.

for BMI-1 antibody (mAb) (Clone DC9)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 32 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide.

for BMI-1 antibody (mAb) (Clone DC9)References

This antibody has been used in the following references:
WB: Bracken AP et al (2007) Genes Dev 21: 535-540.

for BMI-1 antibody (mAb) (Clone DC9)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for BMI-1 antibody (mAb) (Clone DC9)Guarantee

This product is guaranteed for 6 months from date of arrival.