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Antibodies

SIP1 antibody (mAb)

Clone: 6E5
Aliases: Smad-interacting protein 1, aka ZEB2, Zinc finger E-box-binding homeobox 2, ZEB2, ZFHX1B, HSPC082, SMADIP1
Catalog No: 61095 Format: 100 µg $410 Buy Now
Catalog No: 61096 Format: 10 µg $95 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG2a
Purification:Protein G Chromatography
Host:Mouse
Molecular Weight:180 kDa
Reactivity:Human

Applications

Western Blot Validated Immunofluorescence Validated Immunohistochemistry Validated Immunocytochemistry Validated

for SIP1 antibody (mAb) (Clone 6E5)Application Notes


Validated Applications:
WB: 1 - 4 µg/ml dilution

Published Applications:
IHC
ICC/IF
WB
See references for more information. Individual optimization may be required.

for SIP1 antibody (mAb) (Clone 6E5)Immunogen

This SIP1 antibody was raised against a recombinant protein corresponding to amino acids 1-360 of human SIP1.

for SIP1 antibody (mAb) (Clone 6E5)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for SIP1 antibody (mAb) (Clone 6E5)References

 
SIP1 antibody (mAb) tested by Western blot.

Western Blot:
Nuclear extract of Saos-2 cells (20 µg per lane) probed with the SIP1 antibody (mAb) at a dilution of 1 µg/ml.

for SIP1 antibody (mAb) (Clone 6E5)Background

SIP1 (Smad-interacting protein 1, aka ZEB2, Zinc finger E-box-binding homeobox 2) is a sequence-specific DNA binding protein and transcriptional repressor that is involved in normal embryonic development of neural structures and the neural crest. SIP1 belongs to the delta-EF1/Zfh1 family of 2-handed zinc finger/homeodomain proteins and contains a SMAD-binding domain, a homeodomain and two clusters of zinc fingers on the N- and C-termini. SIP1 interacts directly with activated forms of SMAD1, SMAD2 and SMAD3, as well as CBX4 and CTBP1. Mutations in the SIP1 gene cause a form of Hirschsprung disease (HSCR).

for SIP1 antibody (mAb) (Clone 6E5)Positive Control

Active Motif's Saos-2 nuclear extract (Catalog No. 36025) can be used as a positive control for SIP1 antibody.

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with SIP1 antibody (mAb).

for SIP1 antibody (mAb) (Clone 6E5)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for SIP1 antibody (mAb) (Clone 6E5)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

SIP1 antibody (mAb)
 

Data Thumbnails

SIP1 antibody (mAb) tested by Western blot.

Western blot of SIP1 mAb.
(Click image to enlarge and see details.)