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Histone H4 acetyl Lys8 antibody (pAb)
| Catalog No: 61103 | Format: 100 µl | $340 | Buy Now |
| Catalog No: 61104 | Format: 10 µl | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP |
|---|---|
| 0.1 - 1 µg/ml | 5 - 10 µg per ChIP |
for Histone H4 acetyl Lys8 antibody (pAb)Background
Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.
Lysine N-ε-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. The chromatin-remodeling complex SWI/SNF is recruited to promoters through the interaction of the bromodomain of the protein Brg-1, belonging to the SWI/SNF complex, and CBP-acetylated histone H4 Lysine 8, leading to a chromatin remodeling.
for Histone H4 acetyl Lys8 antibody (pAb)Immunogen
This Histone H4 acetyl Lys8 antibody was raised against a peptide containing acetyl Lys8 of human Histone H4.
for Histone H4 acetyl Lys8 antibody (pAb)Application Notes
This Histone H4 acetyl Lys8 antibody has been validated for use in chromatin immunoprecipitation (ChIP, 5 - 10 µg per ChIP) and Western blotting (0.1 - 1 µg/ml).
for Histone H4 acetyl Lys8 antibody (pAb)Buffer
Purified IgG in PBS, 30% glycerol, 0.035% sodium azide.
for Histone H4 acetyl Lys8 antibody (pAb)Positive Control
Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for Histone H3 trimethyl Lys36 antibody.
Chromatin IP Assays
Active Motif has a variety of Chromatin Immunoprecipitation Kits for every need.
Please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H4 acetyl Lys8 antibody (pAb).
Western Blot:
HeLa nuclear extract (20 µg per lane) probed with the Histone H4 acetyl Lys8 antibody (0.5 µg per ml). Lane 1: no treatment. Lane 2: cells treated with sodium butyrate.
Chromatin IP:
ChIP performed using HeLa Chromatin (1.5 x 10^6 cell equivalents per ChIP) and 10 µg of Histone H4 acetyl Lys8 antibody (pAb) or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for either the PABPC1 gene or the GAPDH gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.
Specificity Data:
Dot blot analysis was used to confirm the specificity of the acetyl Lys8 histone H4 antibody. Acetylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at a dilution of 0.1 µg/ml. The amount of peptide (picomoles) spotted is indicated next to each row.
Lane 1: acetyl-Lys5 peptide. Lane 2: acetyl-Lys8 peptide. Lane 3: acetyl-Lys12 peptide. Lane 4: acetyl-Lys16 peptide. Lane 5: acetyl-Lys20 peptide.
for Histone H4 acetyl Lys8 antibody (pAb)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for Histone H4 acetyl Lys8 antibody (pAb)Guarantee
This product is guaranteed for 6 months from date of arrival.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
