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Antibodies

SMRT / NCoR2 antibody (mAb)

Clone: 5.10
Aliases: TRAC, CTG26, SMRTE, TRAC1, N-CoR2, TNRC14, TRAC-1, SMAP270, SMRTE-tau
Catalog No: 61105 Format: 100 µg $410 Buy Now
Catalog No: 61106 Format: 10 µg $95 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG1
Purification:Protein G Chromatography
Host:Mouse
Molecular Weight:300 kDa
Reactivity:Human

Applications

Western Blot Validated

for SMRT / NCoR2 antibody (mAb) (Clone 5.10)Application Notes


Published Applications:
WB
See references for more information. Individual optimization may be required.

for SMRT / NCoR2 antibody (mAb) (Clone 5.10)Immunogen

This SMRT / NCoR2 antibody was raised against a recombinant protein corresponding to amino acids 2004-2310 of human SMRT / NCoR2.

for SMRT / NCoR2 antibody (mAb) (Clone 5.10)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for SMRT / NCoR2 antibody (mAb) (Clone 5.10)References

for SMRT / NCoR2 antibody (mAb) (Clone 5.10)Background

SMRT / NCoR2 (Nuclear receptor corepressor 2, CTG repeat protein 26, SMAP270, Silencing mediator of retinoic acid and thyroid hormone receptor, Thyroid and retinoic-acid-receptor-associated corepressor, TRAC) is a transcriptional corepressor for nuclear receptors. SMRT / NCoR2 has been shown to play a role in regulating immune response, mediating DNA repair, and in neuronal differentiation and cardiac development. SMRT / NCoR2 is part of a large corepressor complex that contains SIN3A/B and histone deacetylases HDAC1 and HDAC2. It also includes TBL1, TBLR1, and GPS2 that help stabilize the overall structure of the complex. SMRT / NCoR2 mediates the transcriptional repression activity of nuclear receptors by promoting histone deacetylation and chromatin condensation, thus preventing access of the basal transcription machinery.

for SMRT / NCoR2 antibody (mAb) (Clone 5.10)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for SMRT / NCoR2 antibody (mAb) (Clone 5.10)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

SMRT / NCoR2 antibody (mAb)