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Histone H1.5 phospho Ser17 antibody (pAb)
| Catalog No: 61107 | Format: 100 µl | $340 | Buy Now |
| Catalog No: 61108 | Format: 10 µl | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP |
|---|---|
| 1:500 - 1:2,000 | — |
for Histone H1.5 phospho Ser17 antibody (pAb)Background
The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA.
Histone H1.5 is an H1 isotype expressed in somatic cells and is heavily phosphorylated during mitosis. Phosphorylation of H1.5 at serine 17 appears in prometaphase and disappears in telophase. The hyperphosphorylated form of H1.5 is mainly chromatin-bound in metaphase when chromatin is maximally condensed. Phosphorylation of H1.5 at serine 17 can be catalyzed by GSK-3.
for Histone H1.5 phospho Ser17 antibody (pAb)Immunogen
This Histone H1.5 phospho Ser17 antibody was raised against a peptide containing phospho Ser17 of human histone H1.5.
for Histone H1.5 phospho Ser17 antibody (pAb)Application Notes
This Histone H1.5 phospho Ser17 antibody has been validated for use in Western blotting (1:500 - 1:2,000 dilution).
For optimal results, primary antibody incubations should be performed overnight at 4°C. Individual optimization may be required. Specificity for histone H1.5 phosphorylated at Serine 17 has been confirmed by dot blot. Due to evolutionary conservation, a wide range of species reactivity is expected.
for Histone H1.5 phospho Ser17 antibody (pAb)Buffer
Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 32 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide.
for Histone H1.5 phospho Ser17 antibody (pAb)Positive Control
Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for Histone H1.5 phospho Ser17 antibody.
Western Blot:
HeLa nuclear extract (20 µg per lane) probed with the Histone H1.5 phospho Ser17 antibody (pAb) at a dilution of 1:1,000.
Specificity Data:
Dot blot analysis was used to confirm the specificity of the Histone H1.5 phospho Ser17 antibody. Peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at a dilution of 1:1,000. The amount of peptide (picomoles) spotted is indicated next to each row.
Lane 1: phospho Ser17 peptide. Lane 2: unmodified Ser17 peptide. Lane 3: phospho Thr17 histone H1.4 peptide.
for Histone H1.5 phospho Ser17 antibody (pAb)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for Histone H1.5 phospho Ser17 antibody (pAb)Guarantee
This product is guaranteed for 6 months from date of arrival.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
