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Antibodies

Histone H1.5S17ph antibody (pAb)

Catalog No: 61107 Format: 100 µl $410 Buy Now
Catalog No: 61108 Format: 10 µl $95 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:36 kDa
Reactivity:Human, Other (Wide Range)

Applications

Western Blot Validated Dot Blot Validated

for Histone H1.5 phospho Ser17 antibody (pAb)Application Notes


Validated Applications:
WB: 1:500 - 1:2,000 dilution

For optimal results, primary antibody incubations should be performed at 4°C. Individual optimization may be required.

for Histone H1.5 phospho Ser17 antibody (pAb)Immunogen

This Histone H1.5 phospho Ser17 antibody was raised against a peptide containing phospho Ser17 of human histone H1.5.

for Histone H1.5 phospho Ser17 antibody (pAb)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for Histone H1.5 phospho Ser17 antibody (pAb)References

 
Histone H1.5S17ph antibody (pAb) tested by Western blot.

Western Blot:
HeLa nuclear extract (20 µg per lane) probed with the Histone H1.5 phospho Ser17 antibody (pAb) at a dilution of 1:1,000.

Histone H1.5S17ph antibody (pAb) tested by dot blot analysis.

Specificity Data:
Dot blot analysis was used to confirm the specificity of the Histone H1.5 phospho Ser17 antibody. Peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at a dilution of 1:1,000. The amount of peptide (picomoles) spotted is indicated next to each row.

Lane 1: phospho Ser17 peptide. Lane 2: unmodified Ser17 peptide. Lane 3: phospho Thr17 histone H1.4 peptide.

for Histone H1.5 phospho Ser17 antibody (pAb)Background

The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA.

Histone H1.5 is an H1 isotype expressed in somatic cells and is heavily phosphorylated during mitosis. Phosphorylation of H1.5 at serine 17 appears in prometaphase and disappears in telophase. The hyperphosphorylated form of H1.5 is mainly chromatin-bound in metaphase when chromatin is maximally condensed. Phosphorylation of H1.5 at serine 17 can be catalyzed by GSK-3.

for Histone H1.5 phospho Ser17 antibody (pAb)Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for Histone H1.5 phospho Ser17 antibody.

for Histone H1.5 phospho Ser17 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Histone H1.5 phospho Ser17 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Histone H1.5S17ph antibody (pAb)
 

Data Thumbnails

Histone H1.5S17ph antibody (pAb) tested by Western blot.

Western blot of Histone H1.5S17ph pAb.
(Click image to enlarge and see details.)

Histone H1.5S17ph antibody (pAb) tested by dot blot analysis.

Dot blot of Histone H1.5S17ph pAb.
(Click image to enlarge and see details.)