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SIP1 antibody (pAb)
| Catalog No: 61113 | Format: 100 µl | $340 | Buy Now |
| Catalog No: 61114 | Format: 10 µl | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP |
|---|---|
| 1:500 - 1:1,000 | — |
for SIP1 antibody (pAb)Background
SIP1 (Smad-interacting protein 1, aka ZEB2, Zinc finger E-box-binding homeobox 2) is a sequence-specific DNA binding protein and transcriptional repressor that is involved in normal embryonic development of neural structures and the neural crest. SIP1 belongs to the delta-EF1/Zfh1 family of 2-handed zinc finger/homeodomain proteins and contains a SMAD-binding domain, a homeodomain and two clusters of zinc fingers on the N- and C-termini. SIP1 interacts directly with activated forms of SMAD1, SMAD2 and SMAD3, as well as CBX4 and CTBP1. Mutations in the SIP1 gene cause a form of Hirschsprung disease (HSCR).
for SIP1 antibody (pAb)Immunogen
This SIP1 antibody was raised against a peptide from the N-terminus of human SIP1.
for SIP1 antibody (pAb)Application Notes
This SIP1 antibody has been validated for use in Western blotting (1:500 - 1:1,000).
for SIP1 antibody (pAb)Buffer
Purified IgG in PBS, 30% glycerol, 0.035% sodium azide.
for SIP1 antibody (pAb)Positive Control
Active Motif's Saos-2 nuclear extract (Catalog No. 36025) can be used as a positive control for SIP1 antibody.
Western blot of SIP1 antibody.
Nuclear extract of Saos-2 cells (20 µg per lane) probed with SIP1 antibody (pAb) at a dilution of 1:500.
for SIP1 antibody (pAb)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for SIP1 antibody (pAb)Guarantee
This product is guaranteed for 6 months from date of arrival.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
