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Antibodies

TIEG1 / KLF10 antibody (pAb)

Aliases: EGRA, TIEG, EGR-alpha
Catalog No: 61117 Format: 100 µl $410 Buy Now
Catalog No: 61118 Format: 10 µl $95 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:50 kDa
Reactivity:Human

Applications

Western Blot Validated

for TIEG1 / KLF10 antibody (pAb)Application Notes


Validated Applications:
WB: 1:500 - 1:1,000 dilution

for TIEG1 / KLF10 antibody (pAb)Immunogen

This TIEG1 / KLF10 antibody was raised against a peptide derived from human TIEG1 / KLF10.

for TIEG1 / KLF10 antibody (pAb)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for TIEG1 / KLF10 antibody (pAb)References

 
TIEG1 / KLF10 antibody (pAb) tested by Western blot.

Western Blot:
K-562 nuclear extract (20 µg per lane) probed with the TIEG1 / KLF10 antibody (pAb) at a dilution of 1:500.

for TIEG1 / KLF10 antibody (pAb)Background

TIEG1 / KLF10 (Transforming growth factor β-inducible early gene 1, Kruppel-like factor 10) is a transcriptional regulator involved in the control of cell growth. KLF10 binds to Sp1 / GC rich DNA sequences and can activate or repress the transcription of a number of genes. KLF10 is especially important for osteoblast function, including bone mineralization and support of osteoclast differentiation. KLF10 is rapidly induced by TGFβ, bone morphogenetic protein, estrogen and epidermal growth factor. KLF10 plays a major role in TGFβ-mediated inhibition of cell proliferation and inflammation and induction of apoptosis.

for TIEG1 / KLF10 antibody (pAb)Positive Control

Active Motif's K-562 nuclear extract (Catalog No. 36015) can be used as a positive control for KLF10 / TIEG1 antibody.

for TIEG1 / KLF10 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for TIEG1 / KLF10 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

TIEG1 / KLF10 antibody (pAb)
 

Data Thumbnails

TIEG1 / KLF10 antibody (pAb) tested by Western blot.

Western blot of TIEG1 / KLF10 pAb.
(Click image to enlarge and see details.)