SMC3 antibody (pAb)

for SMC3 antibody (pAb)Background

The Structural Maintenance of Chromosomes (SMC) family proteins play critical roles in various nuclear events that require structural changes of chromosomes, including mitotic chromosome organization, DNA recombination and repair and global transcriptional repression. SMC3 is a component of the cohesin complex that plays an essential role during chromatid segregation. SMC3 is also involved in DNA recombination and repair, mitotic chromosome organization and microtubule-mediated intracellular transport. SMC3 is acetylated during replication, which is essential for cohesion, and is phosphorylated by ATM subsequent to DNA double strand breakage. SMC3 is frequently elevated in human colon carcinoma and overexpression of the protein transforms fibroblasts.

for SMC3 antibody (pAb)Immunogen

This SMC3 antibody was raised against a peptide in the C-terminal region of human SMC3.

for SMC3 antibody (pAb)Application Notes

This SMC3 antibody has been validated for use in Western blot (1:500 - 1:1,000).

N.B. The addition of Tween is NOT recommended.

for SMC3 antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide.

for SMC3 antibody (pAb)Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for SMC3 antibody.

for SMC3 antibody (pAb)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for SMC3 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of arrival.