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Antibodies

AIRE antibody (pAb)

Aliases: Autoimmune regulator, APECED, Autoimmune polyendocrinopathy candidiasis ectodermal dystrophy, APS1, APSI, PGA1
Catalog No: 61139 Format: 100 µl $410 Buy Now
Catalog No: 61140 Format: 10 µl $95 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:60 kDa
Reactivity:Human

Applications

Western Blot Validated

for AIRE antibody (pAb)Application Notes


Validated Applications:
WB: 1:500 - 1:1,000 dilution

for AIRE antibody (pAb)Immunogen

This AIRE antibody was raised against a peptide in the C-terminal region of human AIRE.

for AIRE antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for AIRE antibody (pAb)References

 
AIRE antibody (pAb) tested by Western blot.

AIRE (pAb) tested by Western blot.
Detection of AIRE by Western blot. The analysis was performed using 30 µg Hep G2 whole-cell extract and AIRE (pAb) at a 1:500 dilution.

for AIRE antibody (pAb)Background

AIRE (Autoimmune regulator, APECED, Autoimmune polyendocrinopathy candidiasis ectodermal dystrophy) is a DNA-binding transcriptional regulator that promotes the expression of tissue-specific self-antigens in the thymus, which is important for self tolerance and the avoidance of autoimmune reactions. AIRE functions as a dimer or as a tetramer and interacts with nucleosomes, selectively interacting with histone H3 unmodified in the N-terminus through its first of two PHD domains. Defects in AIRE are a cause of autoimmune poly-endocrinopathy candidiasis ectodermal dystrophy (APECED) also known as autoimmune polyglandular syndrome type I (APS-1).

for AIRE antibody (pAb)Positive Control

Active Motif's Hep G2 nuclear extract (Catalog No. 36011) can be used as a positive control for AIRE antibody.

for AIRE antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for AIRE antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

AIRE antibody (pAb)
 

Data Thumbnails

AIRE antibody (pAb) tested by Western blot.

Western blot of AIRE pAb.
(Click image to enlarge and see details.)