Active Motif,
Tools to analyze nuclear function,
Your CartYour Cart 0 items
Antibodies

Mili / PiwiL2 antibody (mAb)

Clone: 17.8
Catalog No: 61143 Format: 100 µg $340 Buy Now
Catalog No: 61144 Format: 10 µg $70 Buy Now
Antibody Type:Monoclonal
Host:Mouse
Isotype:IgG1
Purification:Protein G Chromatography
Applications:IF, IHC, IP, WB
Reactivity:Mouse
Molecular Weight:97 kDa

Applications Table

Western Blot Chromatin IP Immunoprecipitation
Published.
See Application Notes below.		 — Published.
See Application Notes below.

for Mili / PiwiL2 antibody (mAb) (Clone 17.8)Background

Mili / PiwiL2 is a member of the PIWI/AGO subfamily of RNA-binding proteins. Mili / PiwiL2 contains a Piwi and an AGO domain and binds a class of RNA called piwi-interacting RNA (piRNA), which are 24 to 30 nucleotides in length and are derived primarily derived from transposons and other repeated sequence elements. Mili / PiwiL2 is required for methylation and inactivation of retrotransposons. Mice deficient in Mili / PiwiL2 are sterile, as it is also required for spermatogenesis. It also positively regulates translation, and is required for germ line stem cell division and differentiation.

for Mili / PiwiL2 antibody (mAb) (Clone 17.8)Immunogen

This Mili / PiwiL2 antibody was raised against full length recombinant mouse MILI / PIWIL2 protein.

for Mili / PiwiL2 antibody (mAb) (Clone 17.8)Application Notes

This Mili / PiwiL2 antibody has been validated for use in immunohistochemistry, immunoprecipitation, immunofluorescence, Western blotting and HITS-CLIP (High-throughput sequencing of RNA isolated by crosslinking immunoprecipitation). See references for more information on specific applications.

for Mili / PiwiL2 antibody (mAb) (Clone 17.8)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide.

Bridging Antibody to Improve ChIP, MeDIP and IP

Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use Mili / PiwiL2 antibody (mAb).

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Mili / PiwiL2 antibody (mAb).

for Mili / PiwiL2 antibody (mAb) (Clone 17.8)References

This antibody has been used in the following references:
IF: Kirino Y et al (2009) Nat Cell Biol 11(5): 652–658.
IP: Kirino Y et al (2009) Nat Cell Biol 11(5): 652–658.
WB: Kirino Y et al (2009) Nat Cell Biol 11(5): 652–658.

for Mili / PiwiL2 antibody (mAb) (Clone 17.8)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Mili / PiwiL2 antibody (mAb) (Clone 17.8)Guarantee

This product is guaranteed for 6 months from date of arrival.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FACS = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • WB = Western Blotting