Notch3 antibody (mAb)

for Notch3 antibody (mAb) (Clone NIZ 8G5)Background

Notch3 is a member of the Notch family of receptors, which make up a highly conserved cell signaling system. Notch3 functions as a ligand-activated transcription factor that directly transduces extracellular signals in the cell surface into changes in gene expression in the nucleus. Notch3 is membrane bound, serving as an extracellular receptor for the Delta and Serrate family of ligands. Upon activation, the intracellular domain is cleaved and rapidly translocates into the nucleus where it forms a transcriptional complex with the RBP-J DNA binding protein and activates gene expression via recruitment of co-activators of the MAML family. Notch receptors play a critical role in cell fate specification during development and participate in multiple biological processes. Notch signaling is required for undifferentiated embryonic stem cells (ESCs) to participate in the formation of all three embryonic germ layers.

for Notch3 antibody (mAb) (Clone NIZ 8G5)Immunogen

This Notch3 antibody was raised against a recombinant protein corresponding to the intracytoplasmic portion of human Notch3.

for Notch3 antibody (mAb) (Clone NIZ 8G5)Application Notes

This Notch3 antibody has been validated for use in Western blotting (2 µg/ml dilution).

for Notch3 antibody (mAb) (Clone NIZ 8G5)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide.

for Notch3 antibody (mAb) (Clone NIZ 8G5)Positive Control

Active Motif's HeLa whole-cell extract (Catalog No. 40050) can be used as a positive control for Notch3 antibody.

for Notch3 antibody (mAb) (Clone NIZ 8G5)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Notch3 antibody (mAb) (Clone NIZ 8G5)Guarantee

This product is guaranteed for 6 months from date of arrival.