Active Motif,
Tools to analyze nuclear function,
Your CartYour Cart 0 items
Antibodies

PIM2 antibody (mAb)

Clone: CHU61
Aliases: Pim-2 Proto-Oncogene, Serine/Threonine Kinase
Catalog No: 61159 Format: 100 µg $410 Buy Now
Catalog No: 61160 Format: 10 µg $95 Buy Now

Request a quote for a bulk orderBulk Order Button


Antibody Type:Monoclonal
Isotype:IgG2a
Purification:Protein G Chromatography
Host:Rat
Molecular Weight:37 kDa
Reactivity:Human

Applications

Western Blot Validated Immunohistochemistry Validated

for PIM2 antibody (mAb) (Clone CHU61)Application Notes


Validated Applications:
IHC: 2 µg/ml dilution
WB: 0.5 - 2 µg/ml dilution

For optimal results, IHC should be performed on paraffin sections. Frozen sections are not recommended.

for PIM2 antibody (mAb) (Clone CHU61)Immunogen

This PIM2 antibody was raised against a recombinant protein corresponding to human PIM2.

for PIM2 antibody (mAb) (Clone CHU61)Buffer

Purified rat IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for PIM2 antibody (mAb) (Clone CHU61)References

 
PIM2 antibody (mAb) tested by immunohistochemistry.

Immunohistochemistry using PIM2 mAb.
PIM2 detected by immunohistochemistry in paraffin-embedded human tonsil cells.

PIM2 antibody (mAb) tested by Western blot.

Western blot of PIM2 antibody (mAb).
Ramos cell extract (100 µg per lane) probed with PIM2 antibody (mAb).

for PIM2 antibody (mAb) (Clone CHU61)Background

PIM2 is a serine/threonine protein kinase in the CAMK family. PIM2 promotes cell survival in response to a variety of proliferative signals through activation of the I-κB kinase/NF-κB pathway. It also inhibits apoptosis through the phosphorylation of BAD. PIM2 has been implicated in the transformation of both T and B lymphocytes and is highly expressed in human leukemia and lymphomas, as well as adenocarcinomas and hepatocellular carcinomas.

for PIM2 antibody (mAb) (Clone CHU61)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for PIM2 antibody (mAb) (Clone CHU61)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

PIM2 antibody (mAb)
 

Data Thumbnails

PIM2 antibody (mAb) tested by immunohistochemistry.

Immunohistochemistry of PIM2 mAb.
(Click image to enlarge and see details.)

PIM2 antibody (mAb) tested by Western blot.

Western blot of PIM2 mAb.
(Click image to enlarge and see details.)