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H3.X/Y antibody (mAb)
| Clone: 8H6-2111 |
| Catalog No: 61161 | Format: 100 µg | $340 | Buy Now |
| Catalog No: 61162 | Format: 10 µg | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP | Immunofluorescence |
|---|---|---|
| Published. See Application Notes below. |
— | Published. See Application Notes below. |
for H3.X/Y antibody (mAb) (Clone 8H6-2111)Background
Histones H3.X and H3.Y are primate-specific variants of histone H3. H3.X and H3.Y are roughly 80% conserved compared to H3.3 H3.Y protein is highly expressed in several primary cell lines, cancer cell lines and human brain. Expression of histone H3.Y is induced by stress. Exogenously expressed H3.X and H3.Y are efficiently incorporated into chromatin and exhibit dynamics similar to canonical histone H3. Knockdown of H3.Y results in slowed growth and altered expression of cell cycle related genes.
for H3.X/Y antibody (mAb) (Clone 8H6-2111)Immunogen
This H3.X/Y antibody was raised against a synthetic peptide corresponding to amino acids 9 - 20 of human H3.X.
for H3.X/Y antibody (mAb) (Clone 8H6-2111)Application Notes
This H3.X/Y antibody recognizes histone variants H3.X and H3.Y and has been validated for use in immunofluorescence, immunohistochemistry and Western blotting. See references for specific details.
for H3.X/Y antibody (mAb) (Clone 8H6-2111)Buffer
Purified rat IgG in PBS with 30% glycerol and 0.035% sodium azide.
Compatible Secondary Antibodies
Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with H3.X/Y antibody (mAb).
for H3.X/Y antibody (mAb) (Clone 8H6-2111)References
This antibody has been used in the following references:
IF: Wiedemann SM et al (2010) J Cell Bio 190: 777-791.
WB: Wiedemann SM et al (2010) J Cell Bio 190: 777-791.
for H3.X/Y antibody (mAb) (Clone 8H6-2111)Storage
Antibodies in solution can be stored at -80°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for H3.X/Y antibody (mAb) (Clone 8H6-2111)Guarantee
This product is guaranteed for 6 months from date of arrival.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
