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Antibodies

Histone H3.X/Y antibody (mAb)

Clone: 8H6-2111
Catalog No: 61161 Format: 100 µg $410 Buy Now
Catalog No: 61162 Format: 10 µg $95 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG2a
Purification:Protein G Chromatography
Host:Rat
Molecular Weight:17 kDa
Reactivity:Human

Applications

Western Blot Validated Immunofluorescence Validated Immunocytochemistry Validated

for Histone H3.X/Y antibody (mAb) (Clone 8H6-2111)Application Notes


Published Applications:
ICC/IF
WB
See references for more information. Individual optimization may be required.

for Histone H3.X/Y antibody (mAb) (Clone 8H6-2111)Immunogen

This H3.X/Y antibody was raised against a synthetic peptide corresponding to amino acids 9 - 20 of human H3.X.

for Histone H3.X/Y antibody (mAb) (Clone 8H6-2111)Buffer

Purified rat IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for Histone H3.X/Y antibody (mAb) (Clone 8H6-2111)References

for Histone H3.X/Y antibody (mAb) (Clone 8H6-2111)Background

Histones H3.X and H3.Y are primate-specific variants of histone H3. H3.X and H3.Y are roughly 80% conserved compared to histone H3.3 The H3.Y protein is highly expressed in several primary cell lines, cancer cell lines and human brain. Expression of histone H3.Y is induced by stress. Exogenously expressed H3.X and H3.Y are efficiently incorporated into chromatin and exhibit dynamics similar to canonical histone H3. Knockdown of H3.Y results in slowed growth and altered expression of cell cycle related genes.

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Histone H3.X/Y antibody (mAb).

for Histone H3.X/Y antibody (mAb) (Clone 8H6-2111)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Histone H3.X/Y antibody (mAb) (Clone 8H6-2111)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Histone H3.X/Y antibody (mAb)