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Antibodies

PRDM1 / BLIMP1 antibody (mAb)

Clone: ROS195
Aliases: PRDI-BF1
Catalog No: 61167 Format: 100 µg $410 Buy Now
Catalog No: 61168 Format: 10 µg $95 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG1
Purification:Protein G Chromatography
Host:Mouse
Molecular Weight:77 & 95 kDa
Reactivity:Human

Applications

Western Blot Validated Immunoprecipitation Validated Immunohistochemistry Validated

for PRDM1 / BLIMP1 antibody (mAb) (Clone ROS195)Application Notes


Published Applications:
IHC
IP
WB
See references for more information. Individual optimization may be required.

for PRDM1 / BLIMP1 antibody (mAb) (Clone ROS195)Immunogen

This PRDM1 / BLIMP1 antibody was raised against full-length recombinant human PRDM1 / BLIMP1 protein.

for PRDM1 / BLIMP1 antibody (mAb) (Clone ROS195)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for PRDM1 / BLIMP1 antibody (mAb) (Clone ROS195)References

for PRDM1 / BLIMP1 antibody (mAb) (Clone ROS195)Background

PRDM1 / BLIMP1 is a transcriptional repressor that binds to specific target sites in DNA and recruits co-repressors. PRDM1 / BLIMP1 is a master regulator for B cell terminal differentiation, is essential for normal development and immunity and a putative tumor suppressor in diffuse large B cell lymphomas (DLBCLs). PRDM1 / BLIMP1 specifies primordial germ cells through the silencing of the default somatic pathway. PRDM1 / BLIMP1 also acts as a master regulator of intestinal maturation.

Bridging Antibody to Improve ChIP, MeDIP and IP

Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use PRDM1 / BLIMP1 antibody (mAb).

for PRDM1 / BLIMP1 antibody (mAb) (Clone ROS195)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for PRDM1 / BLIMP1 antibody (mAb) (Clone ROS195)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

PRDM1 / BLIMP1 antibody (mAb)