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Antibodies

WRN antibody (mAb)

Clone: 195C
Aliases: RECQ3, RECQL2, RECQL3
Catalog No: 61169 Format: 100 µg $410 Buy Now
Catalog No: 61170 Format: 10 µg $95 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG1
Purification:Protein G Chromatography
Host:Mouse
Molecular Weight:162 kDa
Reactivity:Human

Applications

Western Blot Validated Immunofluorescence Validated Immunohistochemistry Validated Immunocytochemistry Validated

for WRN antibody (mAb) (Clone 195C)Application Notes


Validated Applications:
ICC/IF: 0.5 µg/ml dilution
IHC (paraffin): User optimization required
WB: 0.5 - 2 µg/ml dilution

Published Applications:
IHC
WB
See references for more information. Individual optimization may be required.

for WRN antibody (mAb) (Clone 195C)Immunogen

This WRN antibody was raised against a recombinant protein corresponding to amino acids 1072 - 1432 of human WRN.

for WRN antibody (mAb) (Clone 195C)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for WRN antibody (mAb) (Clone 195C)References

 
WRN antibody (mAb) tested by immunofluorescence.

WRN antibody (mAb) tested by Immunofluorescence.
Formaldehyde fixed HeLa cells stained with WRN antibody at a 0.5 µg/ml dilution.

WRN antibody (mAb) tested by immunohistochemistry.

WRN antibody (mAb) tested by Immunohistochemistry.
Paraffin embedded tissue samples: human tonsil, MDA-MB-231 (breast adenocarcinoma cell line), AG11395 WS (fibroblast of a patient with Werner Syndrome immortalized with SV40), HeLa cells, MRC-5 (lung fibroblast cell line), and MCF-7 (breast carcinoma cell line).

WRN antibody (mAb) tested by Western blot.

WRN antibody (mAb) tested by Western blot.
Detection of WRN by Western blot. Lane 1: HeLa cell extract. Lane 2: WS cell extract (fibroblast from a patient with Werner Syndrome).

for WRN antibody (mAb) (Clone 195C)Background

WRN protein is a RECQ helicase that contains 3′ to 5′ helicase activity and DNA-dependent ATPase activity. WRN takes part in many processes, including DNA replication, recombination, repair, and telomere maintenance. Mutations in the gene encoding WRN cause an autosomal recessive segmental progeria called Werner syndrome (WS). WS patients prematurely develop atrophic skin, thin gray hair, osteoporosis, type II diabetes, cataracts, arteriosclerosis and cancer.

for WRN antibody (mAb) (Clone 195C)Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for WRN antibody.

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with WRN antibody (mAb).

for WRN antibody (mAb) (Clone 195C)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for WRN antibody (mAb) (Clone 195C)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

WRN antibody (mAb)
 

Data Thumbnails

WRN antibody (mAb) tested by immunofluorescence.

Immunofluorescence stain of WRN mAb.
(Click image to enlarge and see details.)

WRN antibody (mAb) tested by immunohistochemistry.

Immunohistochemistry of WRN mAb.
(Click image to enlarge and see details.)

WRN antibody (mAb) tested by Western blot.

Western blot of WRN mAb.
(Click image to enlarge and see details.)