Active Motif,
Tools to analyze nuclear function,
Your CartYour Cart 0 items
Antibodies

KAP1 / TIF1β antibody (mAb)

Clone: 1Tb-1A9
Catalog No: 61173 Format: 100 µg $340 Buy Now
Catalog No: 61174 Format: 10 µg $70 Buy Now
Antibody Type:Monoclonal
Host:Mouse
Isotype:IgG2b
Purification:Protein G Chromatography
Applications:ChIP, IF, IP, WB
Reactivity:Human
Molecular Weight:90 kDa

Applications Table

Western Blot Chromatin IP IF IP
Published.
See Application Notes below.		 Published.
See Application Notes below.		 Published.
See Application Notes below.		 Published.
See Application Notes below.

for KAP1 / TIF1β antibody (mAb) (Clone 1Tb-1A9)Background

KAP1 (KRAB Associated Protein 1, TIF1β, Transcriptional Intermediary factor 1β, Tripartite Motif-containing Protein 28, TRIM28, RING finger protein 96, RNF96) is an important co-repressor protein that plays a critical role in proliferation and differentiation of both normal and tumor cells. It functions as a co-repressor for KRAB domain-containing zinc finger proteins (KRAB-ZFPs) and mediates gene silencing by recruiting CHD3, a subunit of the Nucleosome Remodeling and Deacetylation (NuRD) complex. Part of its mechanism involves coordinating the increase in H3K9 methylation at promoters of silenced genes, potentially through direct recruitment of SETDB1, an H3 K9 methyltransferase involved in the formation of heterochromatin. KAP1 / TIF1β is also involved in maintaining pluripotency and is required for terminal differentiation of embryonic stem cells. KAP1 / TIF1β protein levels are increased in liver, gastric, lung, breast, and prostate cancer, and gastric cancer patients with high levels of KAP1 / TIF1β show a significantly lower survival rate. KAP1 / TIF1β contains two zinc fingers and a PHD finger.

for KAP1 / TIF1β antibody (mAb) (Clone 1Tb-1A9)Immunogen

This KAP1 / TIF1β antibody was raised against a full-length protein corresponding to mouse KAP1 / TIF1β.

for KAP1 / TIF1β antibody (mAb) (Clone 1Tb-1A9)Application Notes

This KAP1 / TIF1β antibody has been validated for use in chromatin immunoprecipitation (ChIP), immunofluorescence, immunoprecipitation and Western blotting. See references for more details.

for KAP1 / TIF1β antibody (mAb) (Clone 1Tb-1A9)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide.

Chromatin IP Assays

Active Motif has a variety of Chromatin Immunoprecipitation Kits for every need.
Please visit our Chromatin IP Page to learn about ChIP products designed for use with KAP1 / TIF1β antibody (mAb).

Bridging Antibody to Improve ChIP, MeDIP and IP

Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use KAP1 / TIF1β antibody (mAb).

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with KAP1 / TIF1β antibody (mAb).

for KAP1 / TIF1β antibody (mAb) (Clone 1Tb-1A9)References

This antibody has been used in the following references:
ChIP: Riclet R et al (2009) Mol Biol Cell 20: 296-305.
IF: Nielsen AL et al (1999) EMBO J 18: 6385-6395.
WB: Nielsen AL et al (1999) EMBO J 18: 6385-6395.

for KAP1 / TIF1β antibody (mAb) (Clone 1Tb-1A9)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for KAP1 / TIF1β antibody (mAb) (Clone 1Tb-1A9)Guarantee

This product is guaranteed for 6 months from date of arrival.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FACS = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • WB = Western Blotting