TRIM28 antibody (mAb)

Application Notes

This product has been discontinued:
For Recombinant TRIM28 (624-811) protein, please see Catalog No. 31441


Validated Applications:
ICC/IF: 0.5 µg/ml dilution

Published Applications:
ChIP
ICC/IF
WB
See references for more information. Individual optimization may be required.

Immunogen

This TRIM28 antibody was raised against a full-length protein corresponding to mouse TRIM28.

Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

Background

Tripartite Motif-containing Protein 28 (TRIM28), also known as KAP1 (KRAB Associated Protein 1, and TIF1β, Transcriptional Intermediary factor 1β, is an important co-repressor protein that plays a critical role in proliferation and differentiation of both normal and tumor cells. It functions as a co-repressor for KRAB domain-containing zinc finger proteins (KRAB-ZFPs) and mediates gene silencing by recruiting CHD3, a subunit of the Nucleosome Remodeling and Deacetylation (NuRD) complex. Part of its mechanism involves coordinating the increase in H3K9 methylation at promoters of silenced genes, potentially through direct recruitment of SETDB1, an H3K9 methyltransferase involved in the formation of heterochromatin. TRIM28 is also involved in maintaining pluripotency and is required for terminal differentiation of embryonic stem cells. TRIM28 protein levels are increased in liver, gastric, lung, breast, and prostate cancer, and gastric cancer patients with high levels of TRIM28 show a significantly lower survival rate. TRIM28 contains two zinc fingers and a PHD finger.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT^® High Sensitivity Kit or our magnetic bead-based ChIP-IT^® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with TRIM28 antibody (mAb).

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

• ChIP = Chromatin Immunoprecipitation;
• ChIP = ChIP Sequencing;
• CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
• CUT&Tag = Cleavage Under Targets and Tagmentation;
• DB = Dot Blot;
• ELISA = Enzyme-linked Immunosorbent Assay;
• EMSA = Electrophoretic Mobility Shift Assay
• FC = Flow Cytometry;
• ICC = Immunocytochemistry;
• IF = Immunofluorescence;
• IHC = Immunohistochemistry;
• IP = Immunoprecipitation;
• MeDIP = Methyl-DNA Immunoprecipitation;
• NEU = Neutralizing;
• WB = Western Blotting