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Antibodies

TRIM28 antibody (mAb)

Clone: 1Tb-1A9
Aliases: KAP-1, Tif1b, KRIP-1, MommeD9, AA408787, Tif1beta
Catalog No: 61173 Format: 100 µg $410 Buy Now
Catalog No: 61174 Format: 10 µg $95 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG2b
Purification:Protein G Chromatography
Host:Mouse
Molecular Weight:90 kDa
Reactivity:Human, Mouse

Applications

ChIP Validated Western Blot Validated Immunofluorescence Validated Immunocytochemistry Validated

for TRIM28 antibody (mAb) (Clone 1Tb-1A9)Application Notes


Validated Applications:
ICC/IF: 0.5 µg/ml dilution

Published Applications:
ChIP
ICC/IF
WB
See references for more information. Individual optimization may be required.

for TRIM28 antibody (mAb) (Clone 1Tb-1A9)Immunogen

This TRIM28 antibody was raised against a full-length protein corresponding to mouse TRIM28.

for TRIM28 antibody (mAb) (Clone 1Tb-1A9)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for TRIM28 antibody (mAb) (Clone 1Tb-1A9)References

 
TRIM28 antibody (mAb) tested by immunofluorescence.

TRIM28 antibody (mAb) tested by Immunofluorescence.
Formaldehyde fixed HeLa cells stained with TRIM28 antibody at a 0.5 µg/ml dilution.

for TRIM28 antibody (mAb) (Clone 1Tb-1A9)Background

Tripartite Motif-containing Protein 28 (TRIM28), also known as KAP1 (KRAB Associated Protein 1, and TIF1β, Transcriptional Intermediary factor 1β, is an important co-repressor protein that plays a critical role in proliferation and differentiation of both normal and tumor cells. It functions as a co-repressor for KRAB domain-containing zinc finger proteins (KRAB-ZFPs) and mediates gene silencing by recruiting CHD3, a subunit of the Nucleosome Remodeling and Deacetylation (NuRD) complex. Part of its mechanism involves coordinating the increase in H3K9 methylation at promoters of silenced genes, potentially through direct recruitment of SETDB1, an H3K9 methyltransferase involved in the formation of heterochromatin. TRIM28 is also involved in maintaining pluripotency and is required for terminal differentiation of embryonic stem cells. TRIM28 protein levels are increased in liver, gastric, lung, breast, and prostate cancer, and gastric cancer patients with high levels of TRIM28 show a significantly lower survival rate. TRIM28 contains two zinc fingers and a PHD finger.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with TRIM28 antibody (mAb).

Bridging Antibody to Improve ChIP, MeDIP and IP

Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use TRIM28 antibody (mAb).

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with TRIM28 antibody (mAb).

for TRIM28 antibody (mAb) (Clone 1Tb-1A9)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for TRIM28 antibody (mAb) (Clone 1Tb-1A9)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

TRIM28 antibody (mAb)
 

Data Thumbnails

TRIM28 antibody (mAb) tested by immunofluorescence.

Immunofluorescence stain of TRIM28 mAb.
(Click image to enlarge and see details.)