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LXR-β antibody (pAb)
| Catalog No: 61177 | Format: 100 µl | $340 | Buy Now |
| Catalog No: 61178 | Format: 10 µl | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP | ChIP-Seq / ChIP-chip |
|---|---|---|
| 1:500 - 1:1,000 | 10 µl per ChIP | 10 µl per ChIP-Seq 10 µl per ChIP-chip |
for LXR-β antibody (pAb)Background
LXR-β (Liver X receptor beta, NR1H2, NER, UNR) is an orphan nuclear receptor and a member of the NR1 receptor family. The LXR receptors (including LXR-α and LXR-β ) bind oxysterols and play key roles in maintaining cholesterol homeostasis in macrophages, primarily by regulating multiple components of the reverse cholesterol transport. They also are potent inhibitors of inflammation and are capable of repressing cytokine and chemokine production by Toll-like receptor (TLR)-activated macrophages. LXR-β is more widely expressed than LXR-α, particularly in the brain.
for LXR-β antibody (pAb)Immunogen
This LXR-β antibody was raised against a peptide in the N-terminal region of human LXR-β.
for LXR-β antibody (pAb)Application Notes
This LXR-β antibody has been validated for use in chromatin IP (10 µl per ChIP), ChIP-Seq & ChIP-chip (10 µl each) and Western blotting (1:500 to 1:1,000 dilution).
For optimal results in Western blotting, primary antibody incubations should be performed at 4°C. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.
for LXR-β antibody (pAb)Buffer
Purified rabbit IgG in PBS with 30% glycerol and 0.035% sodium azide.
Chromatin IP Assays
Active Motif has a variety of Chromatin Immunoprecipitation Kits for every need.
Please visit our Chromatin IP Page to learn about ChIP products designed for use with LXR-β antibody (pAb).
LXR-β pAb tested by Western blot.
Detection of LXR-β by Western blot analysis. HeLa whole-cell extract (20 µg) was probed with LXR-β pAb at a 1:1000 dilution.
LXR-β pAb tested by ChIP-qPCR.
Chromatin immunoprecipitation (ChIP) was performed using Active Motif's Epigenetic Services protocol with 25 µg of human liver chromatin and 10 µl of LXR-β antibody. ChIP DNA was used in qPCR with the negative control primer pairs and primers against the known LXR binding site in the RARB promoter. Data are presented as Binding Events Detected per 1000 Cells using Active Motif's Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.
LXR-β pAb tested by ChIP-Chip.
ChIP was performed using chormatin from 2.5 million primary human liver cells. ChIP DNA was amplified by WGA, labeled and hybridized to a human tiling array. The image is zoomed in to show LXR-β binding at a known LXR binding site in the RARB promoter.
for LXR-β antibody (pAb)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for LXR-β antibody (pAb)Guarantee
This product is guaranteed for 6 months from date of arrival.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
