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CENP-B antibody (pAb)
| Catalog No: 61183 | Format: 100 µg | $340 | Buy Now |
| Catalog No: 61184 | Format: 10 µg | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP | Immunofluorescence |
|---|---|---|
| Not recommended. | — | 1 - 2 µg/ml |
for CENP-B antibody (pAb)Background
CENP-B (Centromere protein B) is a protein associated with centromeric DNA and crucial to the formation of the special chromatin structure on the α-satellite DNA repeats of the human centromere. CENP-B specifically binds a 17 bp sequence which may function as a cis-element for centromere-specific nucleosome assembly. CENP-B plays a role in the organization of centromere satellite DNA into a higher order structure, directing centromere formation and kinetochore assembly in mammalian chromosomes.
for CENP-B antibody (pAb)Immunogen
This CENP-B antibody was raised against a recombinant protein corresponding to the C-terminal region of human CENP-B.
for CENP-B antibody (pAb)Application Notes
This CENP-B antibody has been validated for use in immunofluorescence (1 - 2 µg/ml).
for CENP-B antibody (pAb)Buffer
Purified rabbit IgG in PBS with 30% glycerol and 0.035% sodium azide.
Compatible Secondary Antibodies
Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with CENP-B antibody (pAb).
CENP-B pAb tested by immunofluorescence.
Staining of HeLa cells with CENP-B pAb at a 1 µg/ml dilution (green) and α-tubulin (red, Catalog No. 39527) at 1 µg/ml.
for CENP-B antibody (pAb)Storage
Antibodies in solution can be stored at -80°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for CENP-B antibody (pAb)Guarantee
This product is guaranteed for 6 months from date of arrival.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
