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Antibodies

N-Myc antibody (pAb)

Aliases: MYCN, NMYC, ODED, MODED, MYCNOT, bHLHe37
Catalog No: 61185 Format: 100 µl $410 Buy Now
Catalog No: 61186 Format: 10 µl $95 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:60 kDa
Reactivity:Human

Applications

ChIP Validated ChIP-Seq Validated

for N-Myc antibody (pAb)Application Notes


Validated Applications:
ChIP-Seq: 10 - 20 µl per ChIP
WB: 1:500 - 1:2,000 dilution

For optimal results in Western Blot, primary antibody incubations should be performed at 4°C. Individual optimization may be required.

for N-Myc antibody (pAb)Immunogen

This N-Myc antibody was raised against a peptide derived from the C-terminal region of human N-Myc.

for N-Myc antibody (pAb)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for N-Myc antibody (pAb)References

 
N-Myc antibody (pAb) tested by ChIP-Seq.

N-Myc antibody (pAb) tested by ChIP-Seq
Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 200 µg of chromatin from human cell line chromatin and 20 µg N-Myc antibody. ChIP DNA was sequenced on the Illumina HiSeq and 9.2 million sequence tags were mapped to identify N-Myc binding sites on chromosome 3. You can view the complete data set in the UCSC Genome Browser, starting at this specific location, here.

N-Myc antibody (pAb) tested by Western blot.

N-Myc antibody (pAb) tested by Western blot.
Nuclear extract (20 µg) of Kelly cells probed with N-Myc antibody at a dilution of 1:1,000

for N-Myc antibody (pAb)Background

N-Myc (MYCN) is a member of the Myc family of proto-oncogenes. Myc family members play crucial roles in regulating cell proliferation, size and differentiation. N-Myc is required for proper development of the nervous system. Myc family proteins can induce malignant transformation inappropriately modulating gene transcription, leading to unchecked cell proliferation. Misexpression of N-Myc is often associated with pediatric neural cancers such as neuroblastoma, medulloblastoma and retinoblastoma.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with N-Myc antibody (pAb).

for N-Myc antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for N-Myc antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

N-Myc antibody (pAb)
 

Data Thumbnails

N-Myc antibody (pAb) tested by ChIP-Seq.

ChIP-Seq of N-Myc pAb.
(Click image to enlarge and see details.)

N-Myc antibody (pAb) tested by Western blot.

Western blot of N-Myc pAb.
(Click image to enlarge and see details.)