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HNF4A antibody (pAb)
| Catalog No: 61189 | Format: 100 µl | $340 | Buy Now |
| Catalog No: 61190 | Format: 10 µl | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP |
|---|---|
| 1:500 - 1:2,000 | — |
for HNF4A antibody (pAb)Background
HNF4A (hepatocyte nuclear receptor 4A, NR2A1, TCF14) is an orphan nuclear receptor and activator of transcription. HNF4A is major regulator of the hepatocytic phenotype and regulates genes involved in the control of lipid homeostasis. Mutations in HNF4A can cause maturity onset diabetes of the young (MODY1). HNF4A binds DNA as a homodimer and is involved in the development of the liver, kidney and intestine.
for HNF4A antibody (pAb)Immunogen
This HNF4A antibody was raised against a peptide derived from the N-terminal region of human HNF4A.
for HNF4A antibody (pAb)Application Notes
This HNF4A antibody has been validated for use in Western blotting (1:500 - 1:2,000 dilution). For optimal results, primary antibody incubations should be performed at 4°C. Individual optimization may be required.
for HNF4A antibody (pAb)Buffer
Purified IgG in PBS with 30% glycerol and 0.035% sodium azide.
for HNF4A antibody (pAb)Positive Control
Active Motif's Hep G2 whole-cell extract (Catalog No. 36412) can be used as a positive control for HNF4A antibody.
HNF4A antibody (pAb) tested by Western blot.
Whole cell extract (20 µg) of Hep G2 cells probed with HNF4A antibody at a dilution of 1:500.
for HNF4A antibody (pAb)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for HNF4A antibody (pAb)Guarantee
This product is guaranteed for 6 months from date of arrival.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
