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Antibodies

ASPM / MCPH5 antibody (pAb)

Aliases: ASP, MCPH5, Calmbp1
Catalog No: 61205 Format: 100 µg $410 Buy Now
Catalog No: 61206 Format: 10 µg $95 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Protein A Chromatography
Host:Rabbit
Molecular Weight:460 kDa
Reactivity:Human

Applications

Immunofluorescence Validated Immunocytochemistry Validated

for ASPM / MCPH5 antibody (pAb)Application Notes


Validated Applications:
ICC/IF: 2 µg/ml dilution

for ASPM / MCPH5 antibody (pAb)Immunogen

This ASPM / MCPH5 antibody was raised against a peptide within the C-terminal region of human ASPM / MCPH5.

for ASPM / MCPH5 antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for ASPM / MCPH5 antibody (pAb)References

 
ASPM / MCPH5 antibody (pAb) tested by immunofluorescence.

ASPM / MCPH5 antibody tested by immunofluorescence.
Top right: HeLa cells stained with ASPM / MCPH5 antibody (2 µg/ml). Top left: Same cells stained with alpha Tubulin mAb (Clone 5-B-1-2). Bottom left: Stained with DAPI. Bottom right: Merge of all 3 images.

ASPM / MCPH5 antibody (pAb) tested by immunofluorescence.

ASPM / MCPH5 antibody tested by immunofluorescence.
Top right: HeLa cells stained with ASPM / MCPH5 antibody (2 µg/ml). Top left: Same cells stained with alpha Tubulin mAb (Clone 5-B-1-2). Bottom left: Stained with DAPI. Bottom right: Merge of all 3 images.

for ASPM / MCPH5 antibody (pAb)Background

ASPM (abnormal spindle-like microcephaly associated, microcephaly primary type 5, MCPH5) is a protein that participates in spindle organization, spindle positioning and cytokinesis in all dividing cells. ASPM / MCPH5 localizes to centrosomes, spindle poles, and the spindle midbody. It regulates the orientation of the mitotic spindle, which controls whether cells divide symmetrically or asymmetrically. ASPM / MCPH5 is highly expressed in many tumor cell lines and mutations in the gene encoding ASPM / MCPH5 lead to primary microcephaly in humans.

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with ASPM / MCPH5 antibody (pAb).

for ASPM / MCPH5 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for ASPM / MCPH5 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

ASPM / MCPH5 antibody (pAb)
 

Data Thumbnails

ASPM / MCPH5 antibody (pAb) tested by immunofluorescence.

Immunofluorescence stain of ASPM / MCPH5 pAb.
(Click image to enlarge and see details.)

ASPM / MCPH5 antibody (pAb) tested by immunofluorescence.

Immunofluorescence stain of ASPM / MCPH5 pAb.
(Click image to enlarge and see details.)