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ASPM / MCPH5 antibody (pAb)
| Catalog No: 61205 | Format: 100 µg | $340 | Buy Now |
| Catalog No: 61206 | Format: 10 µg | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP | Immunofluorescence |
|---|---|---|
| Not recommended. | — | 2 µg/ml |
for ASPM / MCPH5 antibody (pAb)Background
ASPM (abnormal spindle-like microcephaly associated, microcephaly primary type 5, MCPH5) is a protein that participates in spindle organization, spindle positioning and cytokinesis in all dividing cells. ASPM / MCPH5 localizes to centrosomes, spindle poles, and the spindle midbody. It regulates the orientation of the mitotic spindle, which controls whether cells divide symmetrically or asymmetrically. ASPM / MCPH5 is highly expressed in many tumor cell lines and mutations in the gene encoding ASPM / MCPH5 lead to primary microcephaly in humans.
for ASPM / MCPH5 antibody (pAb)Immunogen
This ASPM / MCPH5 antibody was raised against a peptide within the C-terminal region of human ASPM / MCPH5.
for ASPM / MCPH5 antibody (pAb)Application Notes
This ASPM / MCPH5 antibody has been validated for use in immunofluorescence (IF, 2 µg/ml dilution).
for ASPM / MCPH5 antibody (pAb)Buffer
Purified IgG in PBS with 30% glycerol and 0.035% sodium azide.
Compatible Secondary Antibodies
Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with ASPM / MCPH5 antibody (pAb).
ASPM / MCPH5 antibody tested by immunofluorescence.
Top right: HeLa cells stained with ASPM / MCPH5 antibody (2 µg/ml). Top left: Same cells stained with alpha Tubulin mAb (Clone 5-B-1-2). Bottom left: Stained with DAPI. Bottom right: Merge of all 3 images.
ASPM / MCPH5 antibody tested by immunofluorescence.
Top right: HeLa cells stained with ASPM / MCPH5 antibody (2 µg/ml). Top left: Same cells stained with alpha Tubulin mAb (Clone 5-B-1-2). Bottom left: Stained with DAPI. Bottom right: Merge of all 3 images.
for ASPM / MCPH5 antibody (pAb)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for ASPM / MCPH5 antibody (pAb)Guarantee
This product is guaranteed for 6 months from date of arrival.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
