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Antibodies

FOXG1 antibody (pAb)

Aliases: BF1, BF2, QIN, FKH2, HBF2, HFK1, HFK2, HFK3, KHL2, FHKL3, FKHL1, FKHL2, FKHL3, FKHL4, HBF-1, HBF-2, HBF-3, FOXG1A, FOXG1B, FOXG1C, HBF-G2
Catalog No: 61211 Format: 100 µl $420 Buy Now
Catalog No: 61212 Format: 10 µl $95 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:55 kDa
Reactivity:Human, Mouse

Applications

ChIP Validated ChIP-Seq Validated Western Blot Validated

for FOXG1 antibody (pAb)Application Notes


Validated Applications:
ChIP: 5 µl per ChIP
ChIP-Seq: 5 µl each
WB: 1:500 - 1:1,000 dilution

ChIP-Seq validation was performed by Active Motif's Epigenetics Services and the complete data set is available in the UCSC Genome Browser by clicking here.

For optimal results, primary antibody incubations should be performed at 4°C. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

for FOXG1 antibody (pAb)Immunogen

This FOXG1 antibody was raised against a peptide within the C-terminal region of human FOXG1.

for FOXG1 antibody (pAb)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for FOXG1 antibody (pAb)References

 
FOXG1 antibody (pAb) tested by ChIP-Seq.

FOXG1 antibody (pAb) tested by ChIP-Seq.
ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 30 ug of mouse whole brain chromatin and 2 µl of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 20 million sequence tags were mapped to identify FOXG1 binding sites. The image shows binding across a region of chromosome 4. You can view the complete data set in the UCSC Genome Browser, starting at this specific location, here.

FOXG1 antibody (pAb) tested by Western blot.

FOXG1 antibody (pAb) tested by Western blot.
Detection of Goosecoid by Western blot analysis. Lane 1: Whole-cell extract of Hek 293 cells (40 µg). Lane 2: Whole-cell extract of Jurkat cells (40 µg).
Both were probed with FOXG1 antibody (pAb) at a 1:500 dilution.

for FOXG1 antibody (pAb)Background

FOXG1 (Forkhead box protein G1, FKH2, FKHL1) is a transcriptional repressor that serves an important role in the development of the brain. FOXG1 is expressed in the neural plate early in development and is involved in the specification, proliferation and differentiation of the telencephalon. FOXG1 is also essential for proper patterning of the eye and development of the olfactory epithelium.

for FOXG1 antibody (pAb)Positive Control

Active Motif's Jurkat nuclear extract (Catalog No. 36014) can be used as a positive control for FOXG1 antibody.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with FOXG1 antibody (pAb).

for FOXG1 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for FOXG1 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

FOXG1 antibody (pAb)
 

Data Thumbnails

FOXG1 antibody (pAb) tested by ChIP-Seq.

ChIP-Seq of FOXG1 pAb.
(Click image to enlarge and see details.)

FOXG1 antibody (pAb) tested by Western blot.

Western blot of FOXG1 pAb.
(Click image to enlarge and see details.)