NR2F2 / COUP2 antibody (pAb)
|Catalog No: 61213||Format: 100 µl||$370||Buy Now|
|Catalog No: 61214||Format: 10 µl||$80||Buy Now|
|Western Blot||Chromatin IP||ChIP-Seq /
|1:500 - 1:1,000||5 µl per ChIP||5 µl per ChIP-Seq
5 µl per ChIP-chip
for NR2F2 / COUP2 antibody (pAb)Immunogen
This NR2F2 / COUP2 antibody was raised against a peptide within the N-terminal region of human NR2F2.
for NR2F2 / COUP2 antibody (pAb)Application Notes
This NR2F2 / COUP2 antibody has been validated for use in chromatin IP (ChIP, 5 µl per ChIP), ChIP-Seq & ChIP-chip (5 µl each) and Western blotting (1:500 - 1:1,000 dilution). For optimal results, primary antibody incubations should be performed at 4°C. The addition of 0.1% Tween 20 to all Blotto solutions may reduce background. Individual optimization may be required.
for NR2F2 / COUP2 antibody (pAb)Buffer
Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
for NR2F2 / COUP2 antibody (pAb)Background
NR2F2 / COUP2 (ARP1, TFCOUP2, COT2, COUP-TF2) is a member of the nuclear receptor superfamily related to the COUP transcription factor. It interacts with several nuclear receptor co-activator proteins to activate transcription. NR2F2 / COUP2 plays important roles in signaling pathways in multiple endocrine systems and is crucial for differentiation of the trophoblast during development.
for NR2F2 / COUP2 antibody (pAb)Positive Control
Active Motif's MCF-7 nuclear extract (Catalog No. 36017) can be used as a positive control for NR2F2 / COUP2 antibody.
for NR2F2 / COUP2 antibody (pAb)qPCR Primers
Active Motif's Human Negative Control Primer Set 1 (Cat. No. 71001) has been validated for performing qPCR and endpoint PCR when this antibody has been used for ChIP on human samples.
Chromatin IP Assays
This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with NR2F2 / COUP2 antibody (pAb).
for NR2F2 / COUP2 antibody (pAb)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for NR2F2 / COUP2 antibody (pAb)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
- WB = Western Blotting