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Antibodies

NR2F2 / COUP2 antibody (pAb)

Catalog No: 61213 Format: 100 µl $370 Buy Now
Catalog No: 61214 Format: 10 µl $80 Buy Now
Antibody Type:Polyclonal
Host:Rabbit
Isotype:IgG
Purification:Affinity Purified
Applications:ChIP, ChIP-chip, ChIP-Seq, WB
Reactivity:Human
Molecular Weight:50 kDa

Applications Table

Western Blot Chromatin IP ChIP-Seq /
ChIP-chip
1:500 - 1:1,000 5 µl per ChIP 5 µl per ChIP-Seq
5 µl per ChIP-chip

for NR2F2 / COUP2 antibody (pAb)Immunogen

This NR2F2 / COUP2 antibody was raised against a peptide within the N-terminal region of human NR2F2.

for NR2F2 / COUP2 antibody (pAb)Application Notes

This NR2F2 / COUP2 antibody has been validated for use in chromatin IP (ChIP, 5 µl per ChIP), ChIP-Seq & ChIP-chip (5 µl each) and Western blotting (1:500 - 1:1,000 dilution). For optimal results, primary antibody incubations should be performed at 4°C. The addition of 0.1% Tween 20 to all Blotto solutions may reduce background. Individual optimization may be required.

for NR2F2 / COUP2 antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

 
NR2F2 / COUP2 antibody (pAb) tested by Western blot.

NR2F2 / COUP2 (pAb) tested by Western blot.
Detection of NR2F2 / COUP2 by Western blot. The analysis was performed using 40 µg MCF-7 nuclear extract and NR2F2 / COUP2 (pAb) at a 1:500 dilution.

NR2F2 / COUP2 antibody (pAb) tested by ChIP-Seq.

NR2F2 / COUP2 (pAb) tested by ChIP-Seq.
ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with chromatin from 4.5 million human endometrial stromal cells and 5 µl of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 28 million sequence tags were mapped to identify NR2F2 binding sites. The purple track shows NR2F2 binding across a 1.2 million bp region on chromosome 12. Binding sites showed good correlation with the ENCODE active regulatory elements track (yellow).

for NR2F2 / COUP2 antibody (pAb)Background

NR2F2 / COUP2 (ARP1, TFCOUP2, COT2, COUP-TF2) is a member of the nuclear receptor superfamily related to the COUP transcription factor. It interacts with several nuclear receptor co-activator proteins to activate transcription. NR2F2 / COUP2 plays important roles in signaling pathways in multiple endocrine systems and is crucial for differentiation of the trophoblast during development.

for NR2F2 / COUP2 antibody (pAb)Positive Control

Active Motif's MCF-7 nuclear extract (Catalog No. 36017) can be used as a positive control for NR2F2 / COUP2 antibody.

for NR2F2 / COUP2 antibody (pAb)qPCR Primers

Active Motif's Human Negative Control Primer Set 1 (Cat. No. 71001) has been validated for performing qPCR and endpoint PCR when this antibody has been used for ChIP on human samples.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with NR2F2 / COUP2 antibody (pAb).

for NR2F2 / COUP2 antibody (pAb)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for NR2F2 / COUP2 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FACS = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

NR2F2 / COUP2 antibody (pAb)
 

Data Thumbnails

NR2F2 / COUP2 antibody (pAb) tested by Western blot.

Western blot of NR2F2 / COUP2 pAb.
(Click image to enlarge and see details.)

NR2F2 / COUP2 antibody (pAb) tested by ChIP-Seq.

ChIP-Seq of NR2F2 / COUP2 pAb.
(Click image to enlarge and see details.)