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PHB / Prohibitin antibody (pAb)
| Catalog No: 61217 | Format: 100 µl | $340 | Buy Now |
| Catalog No: 61218 | Format: 10 µl | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP |
|---|---|
| 1:500 - 1:1,000 | — |
for PHB / Prohibitin antibody (pAb)Background
PHB / Prohibitin is a key cell cycle regulatory protein, inhibiting cell division by inactivating E2F family proteins, and has potent anti-proliferative activity. PHB / Prohibitin is also involved in repression of transcription through androgen receptor.
for PHB / Prohibitin antibody (pAb)Immunogen
This PHB / prohibitin antibody was raised against a peptide within the N-terminal region of human PHB.
for PHB / Prohibitin antibody (pAb)Application Notes
This PHB / Prohibitin antibody has been validated for use in Western blotting (1:500 - 1:1,000 dilution). For optimal results, primary antibody incubations should be performed at 4°C. The addition of 0.1% Tween 20 to all Blotto solutions may reduce background. Individual optimization may be required.
for PHB / Prohibitin antibody (pAb)Buffer
Purified IgG in PBS with 30% glycerol and 0.035% sodium azide.
for PHB / Prohibitin antibody (pAb)Positive Control
Active Motif's MCF-7 nuclear extract (Catalog No. 36017) can be used as a positive control for PHB / Prohibitin antibody.
PHB / Prohibitin antibody (pAb) tested by Western blot.
Whole-cell extract of MCF-7 cells (25 µg) probed with PHB antibody (pAb) at a dilution of 1:500.
for PHB / Prohibitin antibody (pAb)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for PHB / Prohibitin antibody (pAb)Guarantee
This product is guaranteed for 6 months from date of arrival.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
