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JMJD2B / KDM4B antibody (pAb)
| Catalog No: 61221 | Format: 100 µl | $340 | Buy Now |
| Catalog No: 61222 | Format: 10 µl | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP |
|---|---|
| 1:500 - 1:1,000 | — |
for JMJD2B / KDM4B antibody (pAb)Background
JMJD2B / KDM4B (JmjC domain-containing histone demethylation protein 3B, Jumonji domain-containing protein 2B, JHDM3B) is a histone demethylase that has been shown to demethylate histone H3 trimethylated at lysine 9. JMJD2B / KDM4B is physically associated with the MLL2 methyltransferase complex where it serves as a co-factor for ERα-mediated transcriptional activation.
for JMJD2B / KDM4B antibody (pAb)Immunogen
This JMJD2B / KDM4B antibody was raised against a peptide within the C-terminal region of human JMJD2B.
for JMJD2B / KDM4B antibody (pAb)Application Notes
This JMJD2B / KDM4B antibody has been validated for use in Western blotting (1:500 - 1:1,000 dilution). For optimal results, primary antibody incubations should be performed at 4°C. The addition of 0.1% Tween 20 to all Blotto solutions may reduce background. Individual optimization may be required.
for JMJD2B / KDM4B antibody (pAb)Buffer
Purified IgG in PBS with 30% glycerol and 0.035% sodium azide.
for JMJD2B / KDM4B antibody (pAb)Positive Control
Active Motif's K-562 nuclear extract (Catalog No. 36015) can be used as a positive control for JMJD2B / KDM4B antibody.
JMJD2B / KDM4B antibody (pAb) tested by Western blot.
Nuclear extract of K-562 cells (40 µg) probed with JMJD2B antibody (pAb) at a dilution of 1:500.
for JMJD2B / KDM4B antibody (pAb)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for JMJD2B / KDM4B antibody (pAb)Guarantee
This product is guaranteed for 6 months from date of arrival.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
