5-Formylcytosine (5-fC) antibody (pAb)
|Catalog No: 61223||Format: 100 µl||$350||Buy Now|
|Catalog No: 61224||Format: 10 µl||$75||Buy Now|
|Western Blot||Chromatin IP||Immunofluorescence|
for 5-Formylcytosine (5-fC) antibody (pAb)Background
DNA methylation is an epigenetic event in which DNA methyltransferases (DNMTs) catalyze the reaction of a methyl group to the fifth carbon of cytosine in a CpG dinucleotide. This modification helps to control gene expression and is also involved in genomic imprinting, while aberrant DNA methylation is often associated with disease. 5-methylcytosine is a modified base that is found in the DNA of plants and vertebrates. A second type of DNA methylation exists, 5-hydroxymethylcytosine (5-hydroxy methylcytosine, 5-hmC). This results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of cytosine oxygenases. Iterative activity of TET on 5-hydroxymethylcytosine results in the conversion of 5-hmC into 5-formylcytosine and 5-carboxylcytosine. An increase in levels of 5-formylcytosine and 5-carboxylcytosine are detected in the mouse male pronucleus following fertilization, which is gradually diluted by DNA replication.
for 5-Formylcytosine (5-fC) antibody (pAb)Immunogen
This 5-Formylcytosine antibody was raised against 5-formylcytidine conjugated to KLH and recognizes 5-formylcytosine.
for 5-Formylcytosine (5-fC) antibody (pAb)Application Notes
This antibody recognizing 5-formylcytosine has been validated for use in dot blot (1:5,000) and immunofluorescence (IF, 1:2,000; see References for details).
for 5-Formylcytosine (5-fC) antibody (pAb)Buffer
Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Compatible Secondary Antibodies
Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with 5-Formylcytosine (5-fC) antibody (pAb).
for 5-Formylcytosine (5-fC) antibody (pAb)References
for 5-Formylcytosine (5-fC) antibody (pAb)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for 5-Formylcytosine (5-fC) antibody (pAb)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
- WB = Western Blotting