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5-Carboxylcytosine (5-caC) antibody (pAb)
| Catalog No: 61225 | Format: 100 µl | $350 | Buy Now |
| Catalog No: 61226 | Format: 10 µl | $75 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP | Immunofluorescence |
|---|---|---|
| — | — | 1:2,000 |
for 5-Carboxylcytosine (5-caC) antibody (pAb)Background
DNA methylation is an epigenetic event in which DNA methyltransferases (DNMTs) catalyze the reaction of a methyl group to the fifth carbon of cytosine in a CpG dinucleotide. This modification helps to control gene expression and is also involved in genomic imprinting, while aberrant DNA methylation is often associated with disease. 5-methylcytosine is a modified base that is found in the DNA of plants and vertebrates. A second type of DNA methylation exists, 5-hydroxymethylcytosine (5-hydroxy methylcytosine, 5-hmC). This results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of cytosine oxygenases. Iterative activity of TET on 5-hydroxymethylcytosine results in the conversion of 5-hmC into 5-formylcytosine and 5-carboxylcytosine. An increase in levels of 5-formylcytosine and 5-carboxylcytosine are detected in the mouse male pronucleus following fertilization, which is gradually diluted by DNA replication.
for 5-Carboxylcytosine (5-caC) antibody (pAb)Immunogen
This 5-Carboxylcytosine antibody was raised against 5-carboxylcytidine conjugated to KLH and recognizes 5-carboxylcytosine.
for 5-Carboxylcytosine (5-caC) antibody (pAb)Application Notes
This antibody recognizing 5-carboxylcytosine has been validated for use in dot blot (1:2,000) and immunofluorescence (IF, 1:2,000; see references for details).
for 5-Carboxylcytosine (5-caC) antibody (pAb)Buffer
Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Compatible Secondary Antibodies
Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with 5-Carboxylcytosine (5-caC) antibody (pAb).
for 5-Carboxylcytosine (5-caC) antibody (pAb)References
The use of this antibody is described in the following references:
DB: Inoue A et al. (2011) Cell Res. 21: 1670-1676.
IF: Inoue A et al. (2011) Cell Res. 21: 1670-1676.
Dot blot of 5-Carboxylcytosine pAb.
Dot blot analysis was used to confirm the specificity of 5-Carboxylcytosine antibody for 5-Carboxylcytidine. Single-stranded DNA oligonucleotides (amount of oligo in nanograms listed on the left side of the blot) corresponding to the immunogen and related sequences were spotted onto nitrocellulose and probed with the antibody at 1:2,000. Lane 1: oligo containing 5-carboxylcytidine. Lane 2: oligo containing 5-formylcytidine. Lane 3: oligo containing 5-hydroxymethylcytidine. Lane 4: oligo containing 5-methylcytidine. Lane 5: oligo containing unmodified cytidine.
for 5-Carboxylcytosine (5-caC) antibody (pAb)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for 5-Carboxylcytosine (5-caC) antibody (pAb)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
- WB = Western Blotting
