BCL11A antibody (mAb)

RRID: AB_2793560
Clone: BCL11A/123
Aliases: EVI9, CTIP1, ZNF856, HBFQTL5, BCL11A-L, BCL11A-S, BCL11a-M, BCL11A-XL
Catalog No: 61231 Format: 100 µg $500 Buy
Catalog No: 61232 Format: 10 µg $125 Buy

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Antibody Type:
Monoclonal
Isotype:
IgG1
Purification:
Protein G Chromatography
Host:
Mouse
Molecular Weight:
100 kDa
Reactivity:
Human

Applications

Western Blot Validated Immunofluorescence Validated Immunohistochemistry Validated Immunocytochemistry Validated

Application Notes

Applications Validated by Active Motif:
WB*: 1 – 2 µg/ml dilution

*Note: we recommend the addition of 0.05% Tween 20 to all blocking solutions to reduce background.

Published Applications

The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:

ICC/IF
IHC
WB

View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.

Immunogen

This BCL11A antibody was raised against a recombinant protein corresponding to amino acids 637-835 of BCL11A-XL (B-cell lymphoma/leukemia 11A extra long form).

Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

 

BCL11A antibody (mAb) tested by Western blot.
HeLa nuclear extract (35 µg per lane) probed with BCL11A antibody at 2 µg/ml dilution.

Background

BCL11A (B-cell CLL/lymphoma 11A) is a transcriptional regulator, a myeloid and B-cell proto-oncogene and plays important roles in leukemogenesis and hematopoiesis. BCL11A is involved in regulating hemoglobin gene expression. BCL11A regulates hemoglobin gene switching during fetal development and silencing of γ-globin expression in adults. BCL11A interacts with the Mi-2/NuRD chromatin remodeling complexes, as well as the erythroid transcription factors GATA1 and FOG1. Abundant expression of full-length forms of BCL11A is developmentally restricted to adult erythroid cells.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting