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Antibodies

EKLF antibody (mAb)

Clone: 7B2
Aliases: KLF1, INLU, CDAN4, HBFQTL6
Catalog No: 61233 Format: 100 µg $410 Buy Now
Catalog No: 61234 Format: 10 µg $95 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG3
Purification:Protein G Chromatography
Host:Mouse
Molecular Weight:42 kDa
Reactivity:Mouse

Applications

ChIP Validated Western Blot Validated Immunoprecipitation Validated

for EKLF antibody (mAb) (Clone 7B2)Application Notes


Published Applications:
ChIP
IP
WB
See references for more information. Individual optimization may be required.

for EKLF antibody (mAb) (Clone 7B2)Immunogen

This EKLF antibody was raised against a full-length recombinant protein to mouse EKLF (Kruppel-like factor 1 (erythroid)).

for EKLF antibody (mAb) (Clone 7B2)Buffer

Purified IgG in 12 mM Tris, pH 8.0, 60 mM Na Citrate, 120 mM NaCl with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for EKLF antibody (mAb) (Clone 7B2)References

for EKLF antibody (mAb) (Clone 7B2)Background

The EKLF (Erythroid krueppel like transcription factor, Erythroid transcription factor, KLF1) gene encodes a hematopoietic-specific transcription factor that induces high-level expression of adult beta-globin and other erythroid genes. The zinc-finger protein binds to the DNA sequence CCACACCCT found in the beta hemoglobin promoter. Furthermore, it binds to the BCL11A promoter and activates expression of BCL11A, which in turn represses the HBG1 and HBG2 genes.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with EKLF antibody (mAb).

Bridging Antibody to Improve ChIP, MeDIP and IP

Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use EKLF antibody (mAb).

for EKLF antibody (mAb) (Clone 7B2)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for EKLF antibody (mAb) (Clone 7B2)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

EKLF antibody (mAb)