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NonO antibody (pAb)
| Catalog No: 61247 | Format: 100 µg | $340 | Buy Now |
| Catalog No: 61248 | Format: 10 µg | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP |
|---|---|
| 0.5 - 2 μg/ml | — |
for NonO antibody (pAb)Background
NonO (non-POU domain containing, octamer-binding, p54(nrb), NRB54, NMT55) is a DNA- and RNA binding protein, involved in several nuclear processes. Binds the conventional octamer sequence in double stranded DNA. Also binds single-stranded DNA and RNA at a site independent of the duplex site. This gene also encodes an RNA-binding protein which plays various roles in the nucleus, including transcriptional regulation and RNA splicing. A rearrangement between this gene and the transcription factor E3 gene has been observed in papillary renal cell carcinoma.
for NonO antibody (pAb)Immunogen
This NonO antibody was raised against a peptide corresponding to the C-terminus of human NonO.
for NonO antibody (pAb)Application Notes
This NonO antibody has been validated for use in Western blot (0.5 - 2 µg/ml).
Addition of 0.05% Tween-20 in blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot.
for NonO antibody (pAb)Buffer
Purified IgG in PBS with 30% glycerol and 0.035% sodium azide.
NonO antibody (pAb) tested by Western blot.
Whole cell extract of HeLa cells (20 µg) probed with NonO antibody (1µg/ml dilution).
for NonO antibody (pAb)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for NonO antibody (pAb)Guarantee
This product is guaranteed for 6 months from date of arrival.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
