5-Methylcytosine (5-mC) antibody (pAb)
|Catalog No: 61255||Format: 100 µg||$350||Buy Now|
|Catalog No: 61256||Format: 10 µl||$75||Buy Now|
for 5-Methylcytosine (5-mC) antibody (pAb)Background
5-Methylcytosine (5-Methylcytidine) is a modified base that is found in the DNA of plants and vertebrates. DNA methylation is an epigenetic event in which DNA methyltransferases (DNMTs) catalyze the reaction of a methyl group to the fifth carbon of cytosine in a CpG dinucleotide. This modification helps to control gene expression and is also involved in genomic imprinting, while aberrant DNA methylation is often associated with disease. The 5-methylcytosine antibody has been developed to discriminate between the modified base and its normal cytosine counterpart, allowing for gene promoter methylation analysis.
for 5-Methylcytosine (5-mC) antibody (pAb)Immunogen
This 5-Methylcytosine (5-mC) antibody was raised against 5-Methyl-cytidine conjugated to KLH and recognizes 5-Methylcytosine.
for 5-Methylcytosine (5-mC) antibody (pAb)Application Notes
This 5-Methylcytosine (5-mC) antibody has been validated for use in applications including dot blot (1:5,000) and methylated DNA immunoprecipitation (MeDIP, 2-10 µg per IP).
for 5-Methylcytosine (5-mC) antibody (pAb)Buffer
Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
for 5-Methylcytosine (5-mC) antibody (pAb)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for 5-Methylcytosine (5-mC) antibody (pAb)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
- WB = Western Blotting