5-Methylcytosine (5-mC) antibody (pAb)

for 5-Methylcytosine (5-mC) antibody (pAb)Background

5-Methylcytosine (5-Methylcytidine) is a modified base that is found in the DNA of plants and vertebrates. DNA methylation is an epigenetic event in which DNA methyltransferases (DNMTs) catalyze the reaction of a methyl group to the fifth carbon of cytosine in a CpG dinucleotide. This modification helps to control gene expression and is also involved in genomic imprinting, while aberrant DNA methylation is often associated with disease. The 5-methylcytosine antibody has been developed to discriminate between the modified base and its normal cytosine counterpart, allowing for gene promoter methylation analysis.

for 5-Methylcytosine (5-mC) antibody (pAb)Immunogen

This 5-Methylcytosine (5-mC) antibody was raised against 5-Methyl-cytidine conjugated to KLH and recognizes 5-Methylcytosine.

for 5-Methylcytosine (5-mC) antibody (pAb)Application Notes

This 5-Methylcytosine (5-mC) antibody has been validated for use in applications including dot blot (1:5,000) and methylated DNA immunoprecipitation (MeDIP, 2-10 µg per IP).

for 5-Methylcytosine (5-mC) antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for 5-Methylcytosine (5-mC) antibody (pAb)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for 5-Methylcytosine (5-mC) antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.