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Antibodies

NKX2.5 antibody (pAb)

Aliases: CSX, CSX1, VSD3, CHNG5, HLHS2, NKX2E, NKX2-5, NKX4-1
Catalog No: 61267 Format: 100 µl $410 Buy Now
Catalog No: 61268 Format: 10 µl $95 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:35 kDa
Reactivity:Human

Applications

Western Blot Validated

for NKX2.5 antibody (pAb)Application Notes


Validated Applications:
WB: 1:500 - 1:1,000 dilution

for NKX2.5 antibody (pAb)Immunogen

This NKX2.5 antibody was raised against a peptide within the N-terminal region of human NKX2.5.

for NKX2.5 antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for NKX2.5 antibody (pAb)References

 
NKX2.5 antibody (pAb) tested by Western blot.

NKX2.5 antibody (pAb) tested by Western blot
Nuclear extract of human heart cells (20 µg) probed with NKX2.5 antibody (1:1,000).

for NKX2.5 antibody (pAb)Background

The NKX2.5 (NK2 homeobox 5, CSX, Homeobox protein CSX, NKX2E, Homeobox protein NK-2 homolog E, NK2 transcription factor related, locus 5, CHNG5, cardiac-specific homeobox 1) gene encodes a homeobox-containing transcription factor that functions in heart formation and development. Alternative splicing results in multiple transcript variants. Implicated in commitment to and/or differentiation of the myocardial lineage. Acts as a transcriptional activator of ANF in cooperation with GATA4. Also interacts with JARID2 which represses its ability to activate transcription of ANF.

for NKX2.5 antibody (pAb)Positive Control

Active Motif's Human Heart nuclear extract (Catalog No. 36041) can be used as a positive control for NKX2.5 antibody.

for NKX2.5 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for NKX2.5 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

NKX2.5 antibody (pAb)
 

Data Thumbnails

NKX2.5 antibody (pAb) tested by Western blot.

Western blot of NKX2.5 pAb.
(Click image to enlarge and see details.)