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Tools to analyze nuclear function,
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Antibodies

NR2C2 antibody (pAb)

Aliases: TR4, TAK1
Catalog No: 61279 Format: 100 µl $410 Buy Now
Catalog No: 61280 Format: 10 µl $95 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:72 kDa
Reactivity:Human

Applications

Western Blot Validated

for NR2C2 antibody (pAb)Application Notes


Validated Applications:
WB: 1:500 - 1:2,000 dilution

for NR2C2 antibody (pAb)Immunogen

This NR2C2 antibody was raised against a peptide within the N-terminal region of human NR2C2.

for NR2C2 antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for NR2C2 antibody (pAb)References

 
NR2C2 antibody (pAb) tested by Western blot.

NR2C2 pAb tested by Western blot.
Detection of NR2C2 by Western blot analysis. Nuclear extract of HEK293 cells (40 µg) was probed with NR2C2 pAb at a 1:500 dilution.

for NR2C2 antibody (pAb)Background

NR2C2 (nuclear receptor subfamily 2, group C, member 2, TR4, TAK1, TR2R1, hTAK1) is an orphan nuclear receptor that can act as a repressor or activator of transcription. An important repressor of nuclear recptor signaling pathways such as retinoic acid receptor, retinoid X, vitamin D3 receptor, thyroid hormone receptor and estrogen receptor pathways. May regulate gene expression during the late phase of spermatogenesis. Together with NR2C1, forms the core of the DRED (direct repeat erythroid-definitive) complex that represses embryonic and fetal globin transcription including that of GATA1. Binds to hormone response elements (HREs) consisting of two 5'-AGGTCA-3' half site direct repeat consensus sequences. Plays a fundamental role in early embryonic development and embryonic stem cells. Required for normal spermatogenesis and cerebellum development. Appears to be important for neurodevelopmentally regulated behavior (By similarity). Activates transcriptional activity of LHCG. Antagonist of PPARA-mediated transactivation.

for NR2C2 antibody (pAb)Positive Control

Active Motif's 293 nuclear extract (Catalog No. 36033) can be used as a positive control for NR2C2 antibody.

for NR2C2 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for NR2C2 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

NR2C2 antibody (pAb)
 

Data Thumbnails

NR2C2 antibody (pAb) tested by Western blot.

Western blot of NR2C2 pAb.
(Click image to enlarge and see details.)