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Antibodies

Histone H4 antibody (pAb)

Catalog No: 61299 Format: 100 µl $410 Buy Now
Catalog No: 61300 Format: 10 µl $95 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:8 kDa
Reactivity:Human, Other (Wide Range)

Applications

ChIP Validated Western Blot Validated

for Histone H4 antibody (pAb)Application Notes


Validated Applications:
ChIP: 2 - 10 µl per ChIP
WB: 1:500 - 1:2,000 dilution

for Histone H4 antibody (pAb)Immunogen

This Histone H4 antibody was raised against a peptide corresponding to amino acids 43- 54 of human histone H4.

for Histone H4 antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for Histone H4 antibody (pAb)References

 
Histone H4 antibody (pAb) tested by ChIP.

Chromatin IP:
ChIP performed using HeLa Chromatin (1.5 x 106 cell equivalents per ChIP) and 10 µl of Histone H4 antibody (pAb) or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the PABPC1 (+ 0.5) gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.

Histone H4 antibody (pAb) tested by Western blot.

Histone H4 antibody (pAb) tested by Western blot
Nuclear extract of HeLa cells (30 µg) probed with Histone H4 antibody (1:1,000).

for Histone H4 antibody (pAb)Background

Histone H4 is one of the core components of the nucleosome, the basic building block of chromatin. Histones are subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.

for Histone H4 antibody (pAb)Positive Control

Active Motif's HeLa acid extract (Catalog No. 36200) can be used as a positive control for Histone H4 antibody.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H4 antibody (pAb).

for Histone H4 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Histone H4 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Histone H4 antibody (pAb)
 

Data Thumbnails

Histone H4 antibody (pAb) tested by ChIP.

ChIP of Histone H4 pAb.
(Click image to enlarge and see details.)

Histone H4 antibody (pAb) tested by Western blot.

Western blot of Histone H4 pAb.
(Click image to enlarge and see details.)