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Antibodies

PITX2 antibody (pAb)

Aliases: Paired Like Homeodomain 2, ALL1-Responsive Protein ARP1, Solurshin, RIEG1
Catalog No: 61303 Format: 100 µl $410 Buy Now
Catalog No: 61304 Format: 10 µl $95 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:33 kDa
Reactivity:Human

Applications

Western Blot Validated

for PITX2 antibody (pAb)Application Notes


Validated Applications:
WB: 1:500 dilution

The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

for PITX2 antibody (pAb)Immunogen

This PITX2 antibody was raised against a peptide within the N-terminal region of human PITX2.

for PITX2 antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for PITX2 antibody (pAb)References

 
PITX2 antibody (pAb) tested by Western blot.

PITX2 antibody (pAb) tested by Western blot.
Whole cell extract of Saos-2 cells (20 µg) probed with PITX2 antibody (1:500).

for PITX2 antibody (pAb)Background

PITX2 (paired-like homeodomain transcription factor 2, ARP1, RGS, RIEG, RIEG1) controls cell proliferation in a tissue-specific manner and is involved in morphogenesis. During embryonic development, exerts a role in the expansion of muscle progenitors. May play a role in the proper localization of asymmetric organs such as the heart and stomach.

for PITX2 antibody (pAb)Positive Control

Active Motif's Saos-2 nuclear extract (Catalog No. 36025) can be used as a positive control for PITX2 antibody.

for PITX2 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for PITX2 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

PITX2 antibody (pAb)
 

Data Thumbnails

PITX2 antibody (pAb) tested by Western blot.

Western blot of PITX2 pAb.
(Click image to enlarge and see details.)