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Antibodies

RNA Pol II CTD phospho Thr4 antibody (pAb)

Aliases: RPB1, RPO2, POLR2, POLRA, RPBh1, RPOL2, RpIILS, hsRPB1, hRPB220
Catalog No: 61307 Format: 100 µg $410 Buy Now
Catalog No: 61308 Format: 10 µg $95 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Protein A Chromatography
Host:Rabbit
Molecular Weight:240 kDa
Reactivity:Human

Applications

Western Blot Validated

for RNA Pol II CTD phospho Thr4 antibody (pAb)Application Notes


Validated Applications:
WB: 1 - 2 µg/ml dilution

The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

for RNA Pol II CTD phospho Thr4 antibody (pAb)Immunogen

This RNA Pol II CTD phospho Thr4 antibody was raised against a peptide containing the RNA Pol II CTD sequence phosphorylated at threonine 4.

for RNA Pol II CTD phospho Thr4 antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for RNA Pol II CTD phospho Thr4 antibody (pAb)References

 
RNA Pol II CTD phospho Thr4 antibody (pAb) tested by Western blot.

RNA pol II CTD phospho Thr4 antibody pAb tested by Western blot.
Nuclear extract of HeLa cells (40 µg) probed with RNA pol II CTD phospho Thr4 antibody at a dilution of 2 µg/ml.

RNA Pol II CTD phospho Thr4 antibody (pAb) tested by dot blot analysis.

RNA Pol II CTD phospho Thr4 pAb tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of RNA Pol II CTD phospho Thr4 pAb. Peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at a dilution of 1 µg/ml. The amount of peptide (picomoles) spotted is indicated next to each row.
    Lane 1: Phospho Thr4 of RNA Pol II CTD peptide.
    Lane 2: Unmodified Thr4 of RNA Pol II CTD peptide.
    Lane 3: Phospho Ser2 of RNA Pol II CTD peptide.
    Lane 4: Phospho Ser5 of RNA Pol II CTD peptide.

for RNA Pol II CTD phospho Thr4 antibody (pAb)Background

RNA pol II (RNA polymerase II) is a complex of 12 proteins that participates in transcription, mRNA processing, and transcription-coupled DNA repair. RNA polymerase II is distinguished by its large carboxyl-terminal repeat domain (CTD), composed of repeats of the consensus heptapeptide Tyr1-Ser2-Pro3-Thr4-Ser5-Pro6-Ser7. Polo-like kinase 3 (plk3) has been reported to phosphorylate Thr4 which may be required for transcriptional elongation.

for RNA Pol II CTD phospho Thr4 antibody (pAb)Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for RNA Pol II CTD phospho Thr4 antibody.

for RNA Pol II CTD phospho Thr4 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for RNA Pol II CTD phospho Thr4 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

RNA Pol II CTD phospho Thr4 antibody (pAb)
 

Data Thumbnails

RNA Pol II CTD phospho Thr4 antibody (pAb) tested by Western blot.

Western blot of RNA Pol II CTD phospho Thr4 pAb.
(Click image to enlarge and see details.)

RNA Pol II CTD phospho Thr4 antibody (pAb) tested by dot blot analysis.

Dot blot of RNA Pol II CTD phospho Thr4 pAb.
(Click image to enlarge and see details.)