Active Motif,
Tools to analyze nuclear function,
Your CartYour Cart 0 items
Antibodies

FOXP1 antibody (mAb)

Clone: 1G1
Catalog No: 61309 Format: 100 µg $410 Buy Now
Catalog No: 61310 Format: 10 µg $95 Buy Now

Request a quote for a bulk orderBulk Order Button


Antibody Type:Monoclonal
Isotype:IgG1
Purification:Protein A Chromatography
Host:Mouse
Molecular Weight:95, 70 & 50kDa
Reactivity:Mouse

Applications

ChIP Validated Western Blot Validated Electrophoretic Mobility Shift Assay Validated

for FOXP1 antibody (mAb) (Clone 1G1)Application Notes


Published Applications:
ChIP
EMSA
WB
See references for more information. Individual optimization may be required.

for FOXP1 antibody (mAb) (Clone 1G1)Immunogen

This FOXP1 antibody was raised against a recombinant protein corresponding to amino acids 495-578 of mouse FOXP1. This antibody detects three isoforms.

for FOXP1 antibody (mAb) (Clone 1G1)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for FOXP1 antibody (mAb) (Clone 1G1)References

for FOXP1 antibody (mAb) (Clone 1G1)Background

FOXP1 (forkhead box P1) is a transcriptional repressor and belongs to subfamily P of the forkhead box (FOX) transcription factor family. It plays an important role in the specification and differentiation of lung epithelium. Can act with CTBP1 to synergistically repress transcription but CTPBP1 is not essential. Essential transcriptional regulator of B-cell development (By similarity).

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with FOXP1 antibody (mAb).

Bridging Antibody to Improve ChIP, MeDIP and IP

Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use FOXP1 antibody (mAb).

for FOXP1 antibody (mAb) (Clone 1G1)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for FOXP1 antibody (mAb) (Clone 1G1)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

FOXP1 antibody (mAb)